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SAB4200743

Sigma-Aldrich

Anti-Human IgG (Fc specific)-Peroxidase antibody, Mouse monoclonal

clone HP-6017, purified from hybridoma cell culture

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Synonym(s):
Anti-Immunoglubulin G (Fc specific)
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

peroxidase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HP-6017, monoclonal

form

lyophilized powder

species reactivity

human

concentration

~2 mg/mL

technique(s)

ELISA: 1:80,000-1:160,000 using 5 μg/mL Human IgG myeloma for coating

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Anti-Human IgG (Fc specific)-Peroxidase antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the HP-6017 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgG myeloma proteins covalently coupled to poly aminostyrene (PAS) microbeads. IgGs (Immunoglobulin G) are the most common Immunoglobulin′s isotype in blood, lymph, cerebrospinal and peritoneal fluids and serve as key players in the humoral immune response. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4), consist of a variable Fab fragment (which includes the antibody recognition site) and a conserved Fc fragment.

Immunogen

Human IgG myeloma proteins

Application

Anti-Human IgG (Fc specific)-Peroxidase antibody has been used in enzyme linked immunosorbent assay (ELISA).

Biochem/physiol Actions

IgG (Immunoglobulin G) deficiencies are often associated with various diseases. The Fc fragment has various important functions (e.g complement fixation, site for rheumatoid factor attachment and protein A binding) which indicate on the importance of immunoreagents specific for the Fc fragment of Human IgG.

Physical form

Supplied as a lyophilized powder.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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