SAB4200746

Anti-α-Fetoprotein (AFP) antibody, Mouse monoclonal

clone C3, purified from hybridoma cell culture

• Synonym(s): Anti-AFP, Anti-Alpha-1-fetoprotein, Anti-Alpha-fetoglobulin
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: C3, monoclonal
• form: buffered aqueous solution
• mol wt: ~74 kDa
• species reactivity: human, canine, porcine
• concentration: ~1.0 mg/mL
• technique(s): ELISA: suitable; dot blot: suitable; immunoblotting: 2-4 μg/mL using liver hepatocellular carcinoma HepG2 cell line extract; immunofluorescence: suitable; immunohistochemistry: 2-4 μg/mL using formalin-fixed, paraffin-embedded human fetal liver sections
• isotype: IgG2a
• UniProt accession no.: P02771
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... AFP(174)

Description

General description

The gene encoding α-fetoprotein (AFP) is localized on human chromosome 4. It encodes a plasma glycoprotein that has a molecular mass of 70kDa. It is synthesized along with serum albumin during the development of embryonic yolk sac and the liver.

Immunogen

Purified human α-fetoprotein

Biochem/physiol Actions

α-fetoprotein (AFP) is involved in the regulation of fatty acids in both fetal and proliferating adult liver cells. Its expression is found to be increased in acute liver injuries, indicating active liver regeneration. AFP has been associated with fatty liver disease (FLD), a disease that may lead to cirrhosis and hepatocellular carcinoma. It serves as a tumor marker for HCC (hepatocellular carcinoma).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Other Notes

In order to obtain best results in different techniques and preparations, we recommend determining optimal working concentration by titration test.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable