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A6684

Sigma-Aldrich

Monoclonal Anti-Albumin antibody produced in mouse

clone HSA-11, ascites fluid

Synonym(s):

Anti-AFP

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

HSA-11, monoclonal

contains

15 mM sodium azide

species reactivity

gibbon, baboon, human, rhesus monkey

should not react with

turkey, feline, hamster, pigeon, rat, marmoset, pig, bovine, canine, donkey, guinea pig, rabbit, chicken, sheep, goat

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: 1:10,000
western blot: 1:5,000

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ALB(213)

General description

Monoclonal Anti-Albumin (mouse IgG2a isotype) is derived from the HSA-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Albumin, the major protein produced by the liver, represents more than half the total protein in human serum. Many other body fluids also contain albumin.
Serum albumin is the major protein component of blood and is produced in the liver. It regulates colloid osmotic blood pressure, transportation of a variety of substances, blood pH and provides an endogenous source of amino acids. Monoclonal anti-albumin antibody (diluted 1:100) can be used in double staining to localize hepatocytes differentiated from human umbilical mesenchymal stem cells (HUMSCs) . Offering specificity for human serum albumin, this product is ideal for identification of human blood stains or other body fluids (e.g., seminal fluid, milk, urine, saliva, vaginal secretions). Mouse monoclonal anti-albumin antibody reacts specifically with denatured and reduced human serum albumin and cross reacts with rhesus, gibbon and baboon serum albumins. The product has shown no cross reactivity with bovine, cat, chicken, dog, donkey, goat, guinea pig, hamster, marmoset, pig, pigeon, rabbit, rat, sheep, or turkey serum albumin or with chicken and turkey egg albumin.

Specificity

The antibody is specific for human albumin in serum and plasma and recognizes an epitope resistant to reduction or denaturation.

Immunogen

Human serum albumin

Application

Monoclonal Anti-Albumin antibody produced in mouse has been used in:
  • immunofluorescence
  • staining
  • double staining
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblot
  • immunohistochemistry

Biochem/physiol Actions

Albumin is a transport protein that binds a broad range of ligands such as fatty acids, bilirubin, hemin, drugs, amino acids and ions . Albumin may also function as a zinc carrier protein and consequently regulate physiological processes .
High levels of albumin are associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia. Oxidative damage of albumin is associated with advanced liver disease.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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