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Key Documents

B2901

Sigma-Aldrich

Monoclonal Anti-Bovine Serum Albumin antibody produced in mouse

clone BSA-33, ascites fluid

Synonym(s):

Anti Bsa, Anti-Bsa Antibody, BSA Antibody - Monoclonal Anti-Bovine Serum Albumin antibody produced in mouse, Bsa Antibody, Anti-Albumin, bovine serum, Anti-BSA

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

BSA-33, monoclonal

contains

15 mM sodium azide

species reactivity

bovine (high), turkey (low), canine (low), sheep (high), horse (low), goat (high)

should not react with

human, hamster, rabbit, donkey, guinea pig, mouse, rat, chicken, feline, pigeon, pig

technique(s)

dot blot: suitable
indirect ELISA: 1:1,000

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

bovine ... Alb(280717)
chicken ... Alb(396197)
dog ... Alb(403550)
horse ... Alb(100034206)
human ... ALB(213)
mouse ... Alb(11657)
pig ... Alb(396960)
rabbit ... Alb(100009195)
rat ... Alb(24186)
sheep ... Alb(443393)

General description

Bovine serum albumin (BSA) is a 66 kDa α-helical, globular and non-glycosylated protein. It is characterized with three domains with two subdomains under each. It belongs to the serum albumins family and has 17-disulfide bonds.

Specificity

Bovine serum albumin (BSA) is the main protein synthesized in the liver that constitutes over 50% of total serum proteins.
Monoclonal Anti-Bovine Serum Albumin antibody is specific for SDS-denatured and reduced BSA. While the product is highly cross reactive with goat and sheep serum; it has a lower affinity for dog, turkey and horse serum albumins.

Immunogen

Bovine serum albumin

Application

Monoclonal Anti-Bovine Serum Albumin has been used in:
  • enzyme linked immunosorbent assays (ELISA)
  • competitive ELISA
  • immunodot blot
  • western blotting
  • immunoaffinity purification or adsorption of BSA from biological fluids.

Biochem/physiol Actions

Bovine serum albumin (BSA) is often used as a protein standard and can also be used in cell culture media, forensics, and foodstuffs preparation. It also acts as a transporter for drugs, hormones and fatty acids.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M A Callahan et al.
The Journal of biological chemistry, 276(30), 28022-28028 (2001-05-29)
During the course of the transmissible spongiform encephalopathy diseases, a protease-resistant ordered aggregate of scrapie prion protein (PrP(Sc)) accumulates in affected animals. From mechanistic and therapeutic points of view, it is relevant to determine the extent to which PrP(Sc) formation
Fatty acids of Treponema pallidum and Borrelia burgdorferi lipoproteins
Belisle JT, et al.
Journal of Bacteriology, 176(2), 2151-2157 (1994)
Marie Louise Gross et al.
Kidney international, 62(1), 51-59 (2002-06-26)
A substantial body of experimental evidence suggests that protein loading causes activation of proximal tubular epithelial cells with consecutive interstitial fibrosis. These studies have mostly been performed using mammalian in vivo models of glomerular damage or tissue cultures of mammalian
Deirdre Thomas-Rudolph et al.
Journal of immunology (Baltimore, Md. : 1950), 178(11), 7283-7291 (2007-05-22)
C-reactive protein (CRP) is an acute phase reactant with roles in innate host defense, clearance of damaged cells, and regulation of the inflammatory response. These activities of CRP depend on ligand recognition, complement activation, and binding to FcgammaR. CRP binds
Molecular identification, immunolocalization, and functional activity of a vacuolar-type H+-ATPase in bovine rumen epithelium
Albrecht E, et al.
Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology, 178(2), 285-295 (2008)

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