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V4630

Sigma-Aldrich

Anti-Vimentin antibody produced in goat

whole antiserum

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Synonym(s):
Anti-F5H288
MDL number:

biological source

goat

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

mol wt

antigen 58 kDa

contains

15 mM sodium azide

species reactivity

wide range, human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
indirect immunofluorescence: 1:20 using formalin-fixed, paraffin-embedded sections of human tissue
western blot: 1:50 using HS-68 human foreskin cell extract

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VIM(7431)

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conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

whole antiserum

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

antibody form

ascites fluid

clone

polyclonal

clone

V9, monoclonal

clone

polyclonal

clone

LN-6, monoclonal

mol wt

antigen 58 kDa

mol wt

antigen ~58 kDa

mol wt

antigen 53 kDa

mol wt

-

species reactivity

wide range, human

species reactivity

hamster, monkey, dog, horse, bovine, rat, gerbil, chicken, pig, feline, human

species reactivity

human, rat, mouse

species reactivity

pig, mouse, sheep, human, bovine, rabbit, feline, rat

General description

The VIM (vimentin) gene codes for an intermediate filament protein, that is expressed throughout mesenchymal cells. VIM gene is mapped to human chromosome 10p13.
Vimentin is encoded by a single-copy gene mapped to human chromosome 10p13. 53kDa vimentin protein belongs to type III intermediate filament (IF) family and is specifically expressed in normal mesenchymal cells. Vimentin protein with 466 amino acids is characterized with a highly conserved α-helical “rod” domain that is flanked by non-α-helical amino- terminal “head” and carboxy-terminal “tail” domain.

Specificity

The antibody localizes vimentin in normal and pathological tissue of mesenchymal derivation. The antibody shows wide cross-reactivity among mammalian species.

Immunogen

vimentin from cultured human foreskin fibroblasts.

Application

Anti-Vimentin antibody produced in goat has been used in Immunohistochemistry and Western blot analysis.
Anti-Vimentin antibody produced in goat has been used in following studies:
  • immunohistochemistry
  • western blotting
  • immunocytochemistry
  • flow cytometry
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunocytochemistry (1 paper)
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)

Biochem/physiol Actions

Vimentin is known to promote epithelial to mesenchymal transition and contributes to metastasis in cancer such as ovarian cancer. Vimentin preserves cellular structure and tissue integrity. Vimentin is also known to bring about TNF-α (tumor necrosis factor-α) induced cell apoptosis.
Vimentin plays a vital role in maintaining the structural integrity of the cells. Overexpression of the gene has been observed in prostate cancer, breast cancer, endometrial cancer, central nervous system (CNS) tumors, malignant melanoma and gastrointestinal tumors including pancreatic, colorectal and hepatic cancers. Thus, vimentin can act as a target for developing therapeutics for treating these cancers. Vimentin plays a crucial role in the vascular endothelial growth factor receptor-1 (VEGFR-1) induced activation of the protein kinase G (PKG) 1 signaling pathway, stimulating regression of cardiomyocyte hypertrophy. Vimentin filaments is implicated in various physiological process including migration, maintenance of cell shape and tolerance of mechanical stress of mesenchymal cells. The protein interacts with LARP6 (la ribonucleoprotein domain family member 6) and stabilizes type I collagen mRNAs and might play a vital role in the development of tissue fibrosis. Vimentin plays an essential role in the maintenance of lens integrity, therefore mutations in the gene causes dominant and pulverulent cataract.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Class VI intermediate filament protein nestin is induced during activation of rat hepatic stellate cells.
Niki T
Hepatology, 29, 520-527 (1999)
Vimentin in cancer and its potential as a molecular target for cancer therapy.
Satelli A and Li S
Cellular and Molecular Life Sciences, 68, 3033-3046 (2011)
Androgen receptor localisation and turnover in human prostate epithelium treated with the antiandrogen, casodex.
Waller A S, et al.
Journal of Molecular Endocrinology, 24(3), 339-351 (2000)
Association of Pasteurella multocida toxin with vimentin.
Shime H, et al.
Infection and Immunity, 70(11), 6460-6463 (2002)
Androgen receptor localisation and turnover in human prostate epithelium treated with the antiandrogen, casodex.
Waller AS
Journal of Molecular Endocrinology, 24, 339-351 (2000)

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