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SAB4200773

Sigma-Aldrich

Anti-Pentraxin 3 antibody, Mouse monoclonal

clone PTX3-5, purified from hybridoma cell culture

Synonym(s):

Pentaxin-related protein PTX3, TNF alpha-induced protein 5, TSG-14, Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor-inducible gene 14 protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

PTX3-5, monoclonal

form

buffered aqueous solution

mol wt

~47 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

immunoblotting: 0.5-1 μg/mL using purified recombinant human Pentraxin 3 protein
immunoprecipitation (IP): 1-2 μg/test using lysate of HEK-293T cells over-expressing human Pentraxin 3

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PTX3(5806)

General description

Anti-Pentraxin 3 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the PTX3-5 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with recombinant human pentraxin 3 protein expressed in HEK-293 cells. Pentraxin 3 (PTX3), also called tumor necrosis factor inducible gene 14 protein (TSG-14), is a highly glycosylated secreted protein usually found as covalently-bound homooctamers. PTX3 belongs to the pentraxin subfamily, sharing the C-terminal domain with short pentraxins and contains a unique N-terminal domain. PTX3 is majorly present in monocytes, macrophages, fibroblasts, endothelial cells and adipocytes. It is an acute-phase protein mapped to human chromosome 3q25.32.

Specificity

Anti-Pentraxin 3 antibody, Mouse monoclonal recognizes both glycosylated and non-glycosylated human PTX3.

Immunogen

recombinant human Pentraxin 3 protein expressed in HEK-293 cells

Application

Anti-Pentraxin 3 antibody, Mouse monoclonal may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Pentraxin 3 (PTX3) is released in response to primary inflammatory signals, such as toll-like receptor engagement, tumor necrosis factor α (TNFα) and interleukin 1β (IL-1β). It regulates innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. PTX3 acts as a soluble pathogen recognition receptor and aids resistance against pathogens. It participates in the removal of apoptotic cells during an immune response. PTX3 associates with complement factor H and associates with synovial fluid in rheumatoid arthritis and also in conditions of sepsis. PTX3 is suppressed by pitavastatin, making it a biomarker for inflammatory cardiovascular disease.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tumor Necrosis Factor Alpha-Induced Proteins in Malignant Tumors: Progress and Prospects
Guo F and Yuan Y
OncoTargets and Therapy, 13, 3303-3303 (2020)
IFN-gamma-inducible protein 10 and pentraxin 3 plasma levels are tools for monitoring inflammation and disease activity in Mycobacterium tuberculosis infection
Azzurri A, et al.
Microbes and Infection, 7(1), 1-8 (2005)
The long pentraxin PTX3 as a link between innate immunity, tissue remodeling, and cancer
Doni A, et al.
Frontiers in Immunology, 10(12), 712-712 (2019)
Pentraxin 3: a novel biomarker for inflammatory cardiovascular disease
Inoue K, et al.
International Journal of Vascular Medicine, 2012 (2012)
Tunicamycin-induced Endoplasmic Reticulum Stress Upregulates the Expression of Pentraxin 3 in Human Retinal Pigment Epithelial Cells
Narae Hwang
Korean Journal of Ophthalmology : KJO, 30(6), 468-478 (2016)

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