SAB4200821

Anti-Fibroblast Surface Protein antibody, Mouse monoclonal

clone 1B10, purified from hybridoma cell culture

• Synonym(s): FSP
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 1B10, monoclonal
• species reactivity: human
• packaging: antibody small pack of 25 μL
• concentration: ~ 1 mg/mL
• technique(s): immunofluorescence: 5-10 μg/mL using Human foreskin fibroblast Hs68 cells.
• isotype: IgM
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... S100A4(6275)

Description

General description

A surface protein characterizing human fibroblasts and fibroblast cell lines, as well as tissue macrophages and 95% of the peripheral blood monocytes. This fibroblast protein antigen has also been demonstrated on malignant fibrosarcoma tissue, human synovial, foreskin and thymic fibroblasts and it is absent from human epithelial cells and lymphocytes.

Specificity

Monoclonal Anti-Human Fibroblast Surface Protein specifically recognizes human fibroblast cells, tissue macrophages and peripheral monocytes .Due to its specific reaction against human fibroblasts, the antibody is useful for removal of fibroblasts from human cultured cells either by inhibition of fibroblasts adherence to the culture vessel or by cytotoxic effect in the presence of rabbit complement.

Immunogen

Cultured human thymic fibroblasts

Application

The antibody is recommended for use in various immunological techniques including Immunofluorescence, flow cytometry, immunohistochemistry and immunoblotting.

Biochem/physiol Actions

Overgrowth of epithelial cells by fibroblasts has long been an obstacle to cultivation of normal and abnormal epithelial cells.Characterization and identification of cells is important for studying functional significance, correlation of cultures with tissue origin, identification of the lineage to which the cells belongs to or identifying the precursor status of the cells.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable