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ZMS1048

Sigma-Aldrich

Anti-Fibroblast Antibody, clone TE-7 ZooMAb® Mouse Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Synonym(s):
Fibrocyte;Fibroblast

biological source

mouse

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

recombinant monoclonal

description

Clone TE-7

product line

ZooMAb® learn more

form

lyophilized

purified by

using protein G

species reactivity

human

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

isotype

IgG1

epitope sequence

Unknown

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

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ZMS1039ZMS1107ZRB1575
recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

clone

recombinant monoclonal

clone

monoclonal, recombinant monoclonal

clone

recombinant monoclonal

clone

2N20, recombinant monoclonal

product line

ZooMAb® learn more

product line

ZooMAb® learn more

product line

ZooMAb® learn more

product line

ZooMAb® learn more

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone TE-7 is a ZooMAb® Mouse recombinant monoclonal antibody that detects fibroblasts in various human tissues.

Immunogen

Human thymic stroma cells.

Application

Quality Control Testing

Evaluated by Immunocytochemistry in human foreskin fibroblasts.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Fibroblast in human foreskin fibroblasts.

Tested applications

Flow Cytometry Analysis: 0.1 μg from a representative lot detected human foreskin fibroblasts.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Fibroblast in human skeletal muscle and human skin tissue sections.

Immunocytochemistry Analysis: A representative lot detected Fibroblast in Immunocytochemistry applications (Goodpaster, T., et. al. (2008). J Histochem Cytochem. 56(4):347-58).

Immunohistochemistry Applications: A representative lot detected Fibroblast in Immunohistohemistry applications (Goodpaster, T., et. al. (2008). J. Histochem. Cytochem. 56(4):347-58).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Fibroblasts, spindle-shaped cells, are derived from undifferentiated mesenchymal cells and are the most common cells of connective tissue in animals. Their main function is to maintain the structural integrity of connective tissues by continuously secreting precursors of ECM. They are capable of both synthesis and degradation of extracellular matrix (ECM). Unstimulated fibroblasts are biosynthetically quiescent, however, when stimulated by extracellular signals they secrete ECM macromolecules, growth factors, and an array of cytokines. They play a critical role in maintaining homeostasis of the microenvironment and play a critical role in wound healing. During the wound healing process, growth factors released by inflammatory cells stimulate fibroblasts to migrate toward a wound where they proliferate and secrete a collagen-rich extracellular matrix. They differentiate into myofibroblasts and actively close the wound by contraction. Clone TE-7 is a reliable marker for mesodermally derived cells in various fetal and adult tissues and fibrosarcoma. It is also useful for the assessment of thymic epithelial culture contamination with fibroblasts. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Goodpaster, T., et al. (2008). J. Histochem. Cytochem. 56(4): 347-358; Haynes, BF., et al. (1984). J. Exp. Med. 159(4); 1149-1168).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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