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SAB4200858

Sigma-Aldrich

Anti-γ-Adaptin antibody, Mouse monoclonal

clone 100/3, purified from hybridoma cell culture

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Synonym(s):
ADTG CLAPG1, adaptor related protein complex 1 subunit gamma 1

antibody form

purified from hybridoma cell culture

Quality Level

clone

100/3, monoclonal

form

liquid

species reactivity

monkey, human, bovine

concentration

~1 mg/mL

isotype

IgG2b

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
A4200A7107SAB4200722
clone

100/3, monoclonal

clone

100/3, monoclonal

clone

A6/2/2, monoclonal

clone

BIII-136, monoclonal

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

isotype

IgG2b

isotype

IgG2b

isotype

IgG1

isotype

IgG2a

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

UniProt accession no.

O43747

UniProt accession no.

O43747

UniProt accession no.

P05549

UniProt accession no.

P02730

General description

Clathrin-mediated endocytosis is a key process in vesicular trafficking that transports various cargo molecules from the plasma membrane of eukaryotic cells into the cytoplasm. These cargoes are involved in various physiological processes, such as cell signaling and developmental regulation.8
Besides clathrin, coated vesicle populations contain the adaptor complexes Assemble Protein 1 (AP1) and Assemble Protein 2 (AP-2), also known as HA-I (HA1) adaptor and HA-II (HA2) adaptor respectively.9 The adaptor proteins are major initiators and regulators of clathrin-coated vesicle trafficking that mediate the recruitment of clathrin to the membranes.8,10

Specificity

Monoclonal Anti- γ-Adaptin antibody specifically recognizes 104 kDa polypeptide1 of the Golgi adaptor complex AP-1 from human, bovine, and monkey6. The antibody reacts with polypeptides of approximately 100 kDa in bovine liver,human heart fibroblasts, and Madin-Darby bovinekidney cultured cells (MDBK).1 The antibody does not recognize the γ-subunit in rodents.1

adaptor complex AP-1

Application

The antibody may be used in various immunochemical techniques including Immunoblot1-6(∼104 kDa), Immunofluorescence1,6, Immunoprecipitation,3 Electron Microscopy7 and Immunoaffinity purification of the Golgi adaptor complex AP-15.

Biochem/physiol Actions

AP2 is responsible for Clathrin-mediated trafficking from the plasma membrane to the endosomes, while AP1 mediates trafficking from the Golgi to endosomes and from the endosomal system to other destinations.10 Biochemical analysis of APs purified from bovine brains determined that they are composed of two ~100 kDa large adaptin subunits (ß1 and γ for AP1, ß2 and α for AP2), one ~50 kDa medium subunit (μ1 for AP1 and μ2 for AP2) which has two folded domains connected by a linker and one ~18 kDa small subunit (σ1 for AP1 and σ2 for AP2).10
Malfunction of clathrin-dependent trafficking pathways have been associated with a variety of human diseases, such as, Alzheimer′s disease, schizophrenia, leukemias and X-linked mental retardation. Additionally, various pathogens take advantage of clathrin-mediated endocytosis to enter cells and use clathrin machinery to promote other aspects of infection.11

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Margaret D Myers et al.
Frontiers in bioscience (Landmark edition), 18, 862-891 (2013-06-12)
Since the identification of clathrin as a vesicular coat protein, numerous studies have contributed to our understanding of the role of clathrin and clathrin-mediated trafficking pathways in cell function. The budding yeast, Saccharomyces cerevisiae, offers a wealth of highly developed
Gwendolyn M Beacham et al.
Traffic (Copenhagen, Denmark), 20(10), 741-751 (2019-07-18)
Heterotetrameric clathrin adaptor protein complexes (APs) orchestrate the formation of coated vesicles for transport among organelles of the cell periphery. AP1 binds membranes enriched for phosphatidylinositol 4-phosphate, such as the trans Golgi network, while AP2 associates with phosphatidylinositol 4,5-bisphosphate of
S Schröder et al.
The Journal of biological chemistry, 266(12), 7910-7918 (1991-04-25)
Clathrin in coated vesicles is linked to transmembrane receptors by adaptor protein complexes. The Golgi-associated adaptor complex HA1 is a tetramer, made up of beta', gamma, 47-kDa, and 20-kDa subunits, whereas the tetrameric plasma membrane adaptor, HA2, contains alpha, beta
S Ahle et al.
The Journal of cell biology, 111(1), 19-29 (1990-07-01)
We have identified a new coat protein in clathrin-coated vesicles from bovine brain by urea-SDS gel electrophoresis. The protein was purified from Tris-solubilized coat proteins either by combination of hydroxyapatite chromatography and gel filtration or more rapidly in a single
Marko Kaksonen et al.
Nature reviews. Molecular cell biology, 19(5), 313-326 (2018-02-08)
Clathrin-mediated endocytosis is a key process in vesicular trafficking that transports a wide range of cargo molecules from the cell surface to the interior. Clathrin-mediated endocytosis was first described over 5 decades ago. Since its discovery, over 50 proteins have

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