SAB4600186
Anti-Rat IgG (H+L), highly cross-adsorbed, CF™ 647 antibody produced in goat
~2 mg/mL, affinity isolated antibody
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Recommended Products
biological source
goat
conjugate
CF™ 647 conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
rat
concentration
~2 mg/mL
technique(s)
flow cytometry: 1-10 μg/mL
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect immunofluorescence: 1-10 μg/mL
fluorescence
λex 650 nm; λem 665 nm
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Related Categories
General description
Immunoglobulin G (IgG) belongs to immunoglobulin family and consists of gamma (γ) heavy chain in the constant (C) region. IgG is a widely expressed serum antibody. Monomeric structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa respectively, the primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2,γ3 and γ4, respectively. IgG is produced and released from CD5+B cells, the predominant lymphocytes in the neonatal cell repertoire. Maternal IgG is the only antibody transported across the placenta to the fetus. Maternal IgG passively immunizes the infants.
CF™ 647 is a cyanine based superior far red dye, with molecular weight of ~836 and has excitation laser line at 633nm, 635nm or 640nm. CF™ 647 is highly water-soluble and pH-insensitive and is brighter and more photostable than Cy™ 5. CF™ 647 has absorption/emission maxima at 650/665nm.
CF™ 647 is a cyanine based superior far red dye, with molecular weight of ~836 and has excitation laser line at 633nm, 635nm or 640nm. CF™ 647 is highly water-soluble and pH-insensitive and is brighter and more photostable than Cy™ 5. CF™ 647 has absorption/emission maxima at 650/665nm.
Specificity
Binds all rat IgGs (minimal cross-reaction with bovine, horse, human and rabbit serum proteins)
Immunogen
rat IgG (H+L)
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Physical form
Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 2 mg/mL bovine serum albumin.
Preparation Note
Protect from light.
Legal Information
This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
6538 is a trademark of American Type Culture Collection
CF is a trademark of Biotium, Inc.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
Certificates of Analysis (COA)
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P Casali et al.
Current topics in microbiology and immunology, 210, 167-179 (1996-01-01)
Natural antibodies arise independently of known antigenic stimulation, are mostly IGM, polyreactive, and are generally encoded by V genes in germline configuration. Polyreactive IgM natural antibodies are produced by mainly B-1 cells which account for most of the B cell
Human placental Fc receptors and the transmission of antibodies from mother to fetus.
Simister NE and Story CM
Journal of Reproductive Immunology, 37(1), 1-23 (1997)
Antibody structure, instability, and formulation.
Wang W
Journal of Pharmaceutical Sciences, 96(1), 1-26 (2007)
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