Skip to Content
MilliporeSigma
All Photos(3)

Key Documents

SAB5600015

Sigma-Aldrich

Anti-Acetyl-Histone H3 (Lys56) antibody, Rabbit monoclonal

recombinant, expressed in HEK 293 cells, clone RM179, purified immunoglobulin

Synonym(s):

H3K56ac, Histone H3 (acetyl K56)

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

recombinant

expressed in HEK 293 cells

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RM179, monoclonal
recombinant monoclonal

form

buffered aqueous glycerol solution

species reactivity

human

concentration

~1 mg/mL

technique(s)

ELISA: 0.5-1 μg/mL
immunocytochemistry: 0.5-2 μg/mL
immunohistochemistry: 1 ug/mL – 10 ug/mL
western blot: 1-2 μg/mL

isotype

IgG

NCBI accession no.

8350

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

acetylation (Lys56)

Gene Information

human ... H3C1(8350)

General description

Mammals contain three main classes of histone H3 variants: the replicative histones (H3.1 and H3.2), the replacement histone (H3.3) and the centromeric histone (Cenp-A). Histone H3 is located on human chromosome 6p22.

Specificity

This antibody reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with other acetylated Lysines in histone H3.

Immunogen

Synthetic peptide corresponding to the Acetyl-Histone H3 (Lys56)

Biochem/physiol Actions

Histone proteins are basic building blocks of chromatin. Histone H3 K27M mutations in adult cerebellar high-grade gliomas. It controls protein-protein interactions to induce binding of trans-acting factors that drive chromatin condensation.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Nataliya Danilenko et al.
Nature communications, 10(1), 3435-3435 (2019-08-08)
Histones, the principal protein components of chromatin, contain long disordered sequences, which are extensively post-translationally modified. Although histone chaperones are known to control both the activity and specificity of histone-modifying enzymes, the mechanisms promoting modification of highly disordered substrates, such
Signaling to chromatin through histone modifications.
Cheung P, et al.
Cell, 103(2), 263-271 (2000)
The program for processing newly synthesized histones H3.1 and H4.
Campos EI
Nature Structural and Molecular Biology, 17(11), 1343-1351 (2010)
Histone H3 K27M mutations in adult cerebellar high-grade gliomas.
Nakata S, et al.
Brain Tumor Pathology, 34(3), 113-119 (2017)
A comprehensive view of the epigenetic landscape part I: DNA methylation, passive and active DNA demethylation pathways and histone variants.
Sadakierska-Chudy A
Neurotoxicity Research, 27(1), 84-97 (2015)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service