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SAE0195

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IceTEV Protease

Histidine tagged recombinant protein, aqueous solution

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Quality Level

form

aqueous solution

shipped in

wet ice

storage temp.

−20°C

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This Item
T445570663P8465
IceTEV Protease Histidine tagged recombinant protein, aqueous solution

SAE0195

IceTEV Protease

TEV Protease

T4455

TEV Protease

Millipore

70663

Proteinase K, Lyophilized

Sigma-Aldrich

P8465

Protease Inhibitor Cocktail powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

-

Quality Level

200

Quality Level

200

Quality Level

400

Quality Level

300

General description

TEV protease is a highly sequence specific serine protease from Tobacco Etch Virus. Its high specificity makes TEV protease popular for cleaving recombinant fusion proteins. However, at low temperatures such as 4 °C, standard TEV Protease has only ~10% of its activity compared to regular temperature conditions (such as 25-30 °C). IceTEV Protease is a new TEV Protease version that is designed to cleave fusion proteins effectively at low temperatures also, in the range of 2-10 °C, with comparable efficiency to cleavage by standard TEV Protease at 25-30 °C. Following the cleavage reaction, IceTEV Protease can be removed from the reaction mixture with nickel-agarose (Ni-agarose) beads.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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Articles

Proteases for biotinylated tag removal for protein purification workflows with related reagents and technical resources.

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