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SMB00610

Sigma-Aldrich

Lipopolysaccharide from Porphyromonas gingivalis

purified by phenol extraction

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Synonym(s):
LPS
UNSPSC Code:
12352211
NACRES:
NA.25

biological source

Porphyromonas gingivalis

Quality Level

form

powder

purified by

phenol extraction

impurities

≤3% Protein (Lowry)

color

white to faint yellow

solubility

triethylene glycol dimethyl ether: 0.90-1.10 mg/mL to hazy, colorless to light yellow

shipped in

ambient

storage temp.

2-8°C

General description

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria like Porphyromonas gingivalis. It differs from LPS from Escherichia coli in its structure and various functional activities.

Application

Lipopolysaccharide has been used:

  • as a treatment to stimulate acute lung injury in human bronchial epithelial (HBE) cells
  • as an agonist for toll-like receptor 4 (TLR4) and to stimulate intestinal normal fibroblasts (NFs) to study osteopontin (OPN) expression in myofibroblasts
  • to study its effects on pro-inflammatory and pro-coagulant genes expression in endothelial cells

Biochem/physiol Actions

LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular patterns (PAMPs).

Additionally, it was demonstrated that the mechanisms by which LPS from E. coli and P. gingivalis modulate cluster of differentiation 14 (CD14), toll-like receptor 2 (TLR2), and toll-like receptor 4 (TLR4) surface expression, primary and secondary cytokine responses are different.

Porphyromonas gingivalis is a Gram-negative bacterium that is known to be involved in adult periodontitis. Periodontitis is a chronic inflammatory disease characterized by the recession of the supportive tissue surrounding teeth. Studies have shown that the LPS from P. gingivalis plays an important role in this disease.
A recent study demonstrated that LPS from P. gingivalis stimulates insulin secretion by the pancreatic β cell line, MIN6. In the presence of 5 mM glucose and 50-500 ng/mL LPS from P. gingivalis, a significant induction of insulin secretion was observed.

Preparation Note

LPS from P. gingivalis is soluble in water and cell culture medium DMEM (1-5 mg/mL), yielding a clear solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Porphyromonas gingivalis Lipopolysaccharide Upregulates Insulin Secretion From Pancreatic β Cell Line MIN6.
Bhat, U.G., et al.
Journal of Periodontology, 85, 1629-1636 (2014)
Periodontal disease.
Williams, R.C.
The New England Journal of Medicine, 322, 373-382 (1990)
M Martin et al.
Journal of immunology (Baltimore, Md. : 1950), 167(9), 5278-5285 (2001-10-24)
Exposure of mononuclear phagocytes to enterobacterial LPS induces a state of transient hyporesponsiveness to subsequent LPS exposure, termed endotoxin tolerance. In the present study, LPS derived from the oral periodontal pathogen, Porphyromonas gingivalis, was compared with that derived from the
Lipopolysaccharide (LPS) of Porphyromonas gingivalis induces IL-1β, TNF-α and IL-6 production by THP-1 cells in a way different from that of Escherichia coli LPS.
Diya, Z., et al.
Innate Immunity, 14, 99-107 (2008)
Differential Induction of Endotoxin Tolerance by Lipopolysaccharides Derived from Porphyromonas gingivalis and Escherichia coli.
Martin, M., et al.
Journal of Immunology, 167, 5278-5285 (2001)

Articles

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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