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SRP0126

Sigma-Aldrich

DNMT1 Active human

recombinant, expressed in baculovirus infected insect cells, ≥50% (SDS-PAGE)

Synonym(s):

CXXC-type zinc finger protein 9, DNA (cytosine-5-)-methyltransferase 1, DNA MTase HsaI, MCMT

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected insect cells

tag

GST tagged (N-terminal)

assay

≥50% (SDS-PAGE)

form

aqueous solution

potency

≥50

mol wt

211 kDa

packaging

pkg of 10 μg

manufacturer/tradename

Sigma-Aldrich

storage condition

avoid repeated freeze/thaw cycles

concentration

0.12 mg/mL

technique(s)

inhibition assay: suitable

NCBI accession no.

UniProt accession no.

application(s)

life science and biopharma

shipped in

dry ice

Storage temp.

−70°C

Gene Information

human ... DNMT1(1786)

General description

Research area: Cell cycle

DNMT1 (DNA methyltransferase 1) is a member of the DNMT enzyme family which also includes DNMT2, DNMT3A, and DNMT3B. It is a multidomain protein composed of 1616 amino acids. It has a methyltransferase domain in its C-terminal, which shows sequence homology to bacterial methyltransferases. The DNMT1 gene is mapped to human chromosome 19p13.2.
The DNMT1 gene is mapped to human chromosome 19p13.2.

Application

DNMT1 (DNA methyltransferase 1) has been used for RNA electrophoretic mobility shift assay (REMSA) to determine the role of extra-coding RNAs (ecRNAs) in controlling neuronal DNA methylation, through interaction with DNA methyltransferases.
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

DNMT1 (DNA methyltransferase 1) is the predominant enzyme that is responsible for maintaining the level of DNA methylation. It is responsible for replicating CpG methylation patterns from parent to daughter DNA strands, thus, creating heritable methylation signatures through cell division. It, therefore, maintains epigenetic state of DNA. Abnormalities in the expression of this protein are linked with the progression and prognosis of multiple cancers, such as hepatocellular carcinoma, pancreatic, lung and bladder cancers. Up-regulation of this protein in GC (gastric cancer) with less differentiation, advanced stage and increased rate of mortality. Upregulation of the DNMT1 gene indicates poor prognosis in malignant cancers including renal cell carcinoma, lymphoma, pancreatic and and bladder cancer.

Unit Definition

One unit is defined as the amount of enzyme required to methylate 1 pmol of DNA substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Differential expression of the epigenetic methylation-related protein DNMT1 by breast cancer molecular subtype and stromal histology.
Shin E, et al.
Journal of Translational Medicine, 14:87 (2016)
Epigenetic regulation of DNA methyltransferases: DNMT1 and DNMT3B in gliomas.
Rajendran G, et al.
Journal of Neuro-Oncology, 104(2), 483-494 (2011)
Toru Kawakami et al.
Journal of peptide science : an official publication of the European Peptide Society, 25(9), e3200-e3200 (2019-07-17)
Posttranslational modifications of histone proteins, which form nucleosome cores, play an important role in gene regulation. Ubiquitination is one such modification. We previously reported on the synthesis of ubiquitinated histone H3 with an isopeptide mimetic structure. In this report, we
Polymorphisms of the DNA Methyltransferase 1 Gene Predict Survival of Gastric Cancer Patients Receiving Tumorectomy.
Jia Z, et al.
Disease Markers (2016)
Hiroki Kimura et al.
Journal of immunology (Baltimore, Md. : 1950), 199(11), 3870-3882 (2017-10-27)
Appropriate resolution of inflammation is known to be essential in tissue homeostasis. In this study, we evaluated the significance of a macrophage-derived soluble protein, apoptosis inhibitor of macrophage (AIM), in LPS-induced lung injury in mice. After oropharyngeal administration of LPS

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