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SRP0356

Sigma-Aldrich

GSK3β active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥80% (SDS-PAGE)

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Synonym(s):
glycogen synthase kinase 3 beta
CAS Number:
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

assay

≥80% (SDS-PAGE)

form

aqueous solution

specific activity

≥500 pmol/min-μg

mol wt

73 kDa

packaging

pkg of 10 μg

technique(s)

activity assay: suitable
inhibition assay: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... GSK3B(2932)

General description

Glycogen Synthase Kinase 3β (GSK3β) is one of the two isoforms of GSK3 enzyme, both of which are encoded by different genes. This protein is predominantly cytoplasmic, but is also found to be localized to nucleus and mitochondria. It is a protein-serine kinase. This protein contains a protein kinase domain in its core, and is composed of 482 amino acids with a molecular weight of 46,712Da. It shows a wide range of tissue expression with the highest expression in brain.
Human GSK3beta GenBank Accession No. NM_002093), full length with N-terminal GST-tag, MW=73 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Glycogen Synthase Kinase 3β (GSK3β) is responsible for rapid cyclin D1 turnover by phosphorylating it on Thr-286. It functions as a negative regulator of Wnt signaling during embryonic development and cell proliferation in adult tissues. This protein phosphorylates and regulates multiple proteins such as glycogen synthase, insulin receptor substrate-1, axin and APC (adenomatous polyposis coli). This protein is also shown to interact with multiple protein which are linked with Alzheimer′s disease (AD).

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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