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Key Documents

SRP3013

Sigma-Aldrich

Beta D-4 human

recombinant, expressed in E. coli, ≥98% (HPLC), suitable for cell culture

Synonym(s):

Beta-defensin 104, DEFB104A

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)

form

lyophilized

mol wt

6.0 kDa

packaging

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

suitability

suitable for

UniProt accession no.

Q8WTQ1

antibiotic activity spectrum

fungi
mycobacteria
viruses

mode of action

cell membrane | interferes

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... DEFB104A(140596)

General description

Chemical structure: peptide
Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, four human b-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence. b-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. β-defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. BD-4 is expressed in testis, stomach, uterus, neutrophils, thyroid, lung and kidney. In addition to its direct antimicrobial activities, BD-4 is chemoattractant towards human blood monocytes. Recombinant human BD-4 is a 6.0 kDa protein containing 50 amino acid residues.

Biochem/physiol Actions

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. Recombinant human BD-4 is a 6.0 kDa protein containing 50 amino acid residues.

Sequence

EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP

Physical form

Lyophilized from 10 mM Acetic Acid.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in 10 mM Acetic Acid to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Y Q Tang et al.
Science (New York, N.Y.), 286(5439), 498-502 (1999-10-16)
Analysis of rhesus macaque leukocytes disclosed the presence of an 18-residue macrocyclic, tridisulfide antibiotic peptide in granules of neutrophils and monocytes. The peptide, termed rhesus theta defensin-1 (RTD-1), is microbicidal for bacteria and fungi at low micromolar concentrations. Antibacterial activity
Robert I Lehrer et al.
Current opinion in immunology, 14(1), 96-102 (2002-01-16)
During the past year, novel beta-defensins of mice and men have been identified, together with a novel defensin subfamily (the circular or 'theta' minidefensins) in primates. Insight into the evolution of defensins has been obtained from structural studies, and several

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