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SRP3055

Sigma-Aldrich

Heregulin beta -1 human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

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Synonym(s):
NRG1-b1, Neuregulin1
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.5 ng/mL

mol wt

7.5 kDa

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... NRG1(3084)

General description

Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). HRG1-β1 (heregulin-β1, also referred to as NRG1-β1) contains an Ig (immunoglobulin) domain and an EGF (epidermal growth factor)-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. The gene HRG1-β1 is mapped to human chromosome 8p12. Recombinant human Heregulin-β1 is a 7.5 kDa polypeptide consisting of only the EGF domain of heregulin-β1 (65 amino acid residues).

Application

Heregulin beta -1 human has been used to enhance proliferation of human dermal fibroblasts (hDFs).
Heregulin-β1 human has been used in the culture medium for the maintenance of Schwann cells. It has been used to study P-Rex1 (PI(3,4,5)P3-dependent Rac exchanger 1)-driven fibroblast invasiveness.

Biochem/physiol Actions

To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 (neuregulin1) gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms release soluble growth factors. HRG1-β1 (heregulin-β1, also referred to as NRG1-β1) binding to receptor tyrosine kinases erb3 and erb4 induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity, which leads to tyrosine phosphorylation. Although HRG1-β1 biological effects is still unclear, it has been found to promote motility and invasiveness of breast cancer cells which may also involve up-regulation of expression and function of the autocrine motility-promoting factor (AMF).

Sequence

SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Enhancement of wound healing efficiency mediated by artificial dermis functionalized with EGF or NRG1
Yoon D, et al.
Biomedical Materials (Bristol, England), 13(4), 045007-045007 (2018)
Control of peripheral nerve myelination by the beta-secretase BACE1.
Willem M
Science, 314, 664-666 (2006)
Neuregulins and cancer.
Montero JC
Clinical Cancer Research, 14, 3237-3241 (2008)
Neuregulins: functions, forms, and signaling strategies.
Falls DL
Experimental Cell Research, 284, 14-30 (2003)
P-Rex1 cooperates with PDGFR? to drive cellular migration in 3D microenvironments.
P-Rex1 cooperates with PDGFR? to drive cellular migration in 3D microenvironments.
Campbell AD
PLoS ONE, 8, e53982-e53982 (2013)

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