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SRP3153

Sigma-Aldrich

SCGF-beta human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

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Synonym(s):
Stem Cell Growth Factor-beta
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤7.0 ng/mL ED50

mol wt

25.0 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CLEC11A(6320)

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vibrant-m

SRP3153

SCGF-beta human

vibrant-m

SRP3152

SCGF-alpha human

vibrant-m

SRP3234

SCF from mouse

biological source

human

biological source

human

biological source

human

biological source

mouse

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

>98% (SDS-PAGE and HPLC)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

packaging

pkg of 10 μg

packaging

pkg of 10 μg

packaging

-

packaging

pkg of 10 μg

General description

SCGF alpha and beta are hematopoietic growth factor that exerts their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively. Recombinant human SCGF-b is a 25.0 kDa polypeptide containing 227 amino acid residues.

Biochem/physiol Actions

SCGF alpha and beta are hematopoietic growth factor that exerts their activity at early stages of hematopoiesis. Recombinant human SCGF-b is a 25.0 kDa polypeptide containing 227 amino acid residues.

Sequence

MGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ PSASPHPLSP DQPNGGTLEN CVAQASDDGS WWDHDCQRRL YYVCEFPF

Physical form

Lyophilized from 0.25x PBS + 1mM DTT.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Xu Ding et al.
Fish & shellfish immunology, 33(3), 494-503 (2012-06-12)
The toll-like receptors (TLRs) are an important gene family in host innate immunologic surveillance. The TLR22 gene is an essential member of the TLRs that is only found in aquatic animals and has been detected in some bony fish. Here

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Read article on hematopoietic cytokines and hematopoiesis

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