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SRP3327

Sigma-Aldrich

IL-1 RECEPTOR ANTAGONIST human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

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Synonym(s):
ICIL-1RA, IL-1RN, IL-1Ra, IRAP
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

20-40 ng/mL

mol wt

17.2 kDa

packaging

pkg of 100 μg

storage condition

(Keep container tightly closed in a dry and well-ventilated place)

technique(s)

activity assay: suitable

impurities

endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL1R1(3554)

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SRP3327

IL-1 RECEPTOR ANTAGONIST human

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SRP6006

IL-1RA murine

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SRP3320

IL-36RA human

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

>95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

technique(s)

activity assay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

recombinant

expressed in E. coli

recombinant

expressed in HEK 293 cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage condition

(Keep container tightly closed in a dry and well-ventilated place)

storage condition

avoid repeated freeze/thaw cycles

storage condition

-

storage condition

-

General description

Research area: Immunology & cytokines

Interleukin-1 receptor antagonist (IL-1RA) is a naturally occurring inflammatory inhibitor protein. IL-1Ra is produced by corneal epithelial cells, monocytes, neutrophils, macrophages, and fibroblasts. Recombinant human IL-1Ra is a 17.2kDa protein consisting of 153 amino acid residues.

Application

Interleukin-1 receptor antagonist (IL-1RA) human has been used:
  • to study the role of interleukin (IL)-1β receptor in cell senescence induced by doxorubicin
  • to block the IL-1β binding to its receptor
  • to understand the role of autocrine interleukin-1β (IL-1β) signaling downstream of cl- in RPS27 modulation

Biochem/physiol Actions

Interleukin-1 receptor antagonist (IL-1RA) inhibits the activity of IL-1α and IL-1β by competitively blocking binding to their type I and type II receptors. Therapeutically, IL-1RA may help in the treatment of sepsis, cachexia, rheumatoid arthritis, chronic myelogenous leukemia, asthma, psoriasis, and inflammatory bowel disease.

Physical form

Lyophilized from 20 mM Tris, pH 8.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Exposure of T lymphocytes to leflunomide but not to dexamethasone favors the production by monocytic cells of interleukin-1 receptor antagonist and the tissue-inhibitor of metalloproteinases-1 over that of interleukin-1beta and metalloproteinases.
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International Journal of Clinical and Experimental Pathology, 8, 4182-4185 (2015)

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