Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

View All Documentation

SRP5232

Sigma-Aldrich

EIF2S1, His tagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

EIF-2, EIF-2α, EIF-2A, EIF2, EIF2A

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
480740-00-9
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

~40 kDa

NCBI accession no.

NM_004094

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... EIF2S1(1965)

General description

Eukaryotic translation initiation factor 2 subunit 1 α (EIF2S1) is a 35kDa protein. It is the α subunit of the eukaryotic initiation factor 2 (eIF2α). The gene encoding it is localized on human chromosome 14q23.3.

Biochem/physiol Actions

Eukaryotic translation initiation factor 2 subunit 1 α (EIF2S1) catalyzes the first regulated step of protein synthesis initiation and promotes the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. The Ser51 in the mature human EIF2-α protein acts as the sole site of phosphorylation that leads to repression of protein synthesis. EIF2-α also plays an essential role in erythropoiesis.

Physical form

Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Ppp1r15 gene knockout reveals an essential role for translation initiation factor 2 alpha (eIF2a) dephosphorylation in mammalian development
Heather P
Proceedings of the National Academy of Sciences of the USA (2008)
Direct interaction between STAT3 and EIF2AK2 controls fatty acid-induced autophagy
Mireia N
Autophagy (2012)
ER stress and autophagy contribute to CsA-induced death of malignant glioma cells
Iwona Anna
Autophagy (2012)
Mechanism of activation of the double-stranded-RNA-dependent protein kinase, PKR
Krishna m
FEBS Journal (2001)
A SNP microarray and FISH-based procedure to detect allelic imbalances in multiple myeloma: An integrated genomics approach reveals a wide gene dosage effect
Luca A
Genes (2009)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service