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SRP5335

Sigma-Aldrich

PP2C-α, active, GST tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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Synonym(s):
FLJ42306, MGC9201, PP2C-ALPHA, PP2CA, PPM1A
CAS Number:
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

960-1440 nmol/min·mg

mol wt

~105 kDa

NCBI accession no.

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PPM1A(5494)

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vibrant-m

SRP5335

PP2C-α, active, GST tagged human

vibrant-m

SRP6062

PP2C α human

vibrant-m

SRP5334

PP2C-γ, active, GST tagged human

vibrant-m

SRP5338

PP1A, active, GST tagged human

biological source

human

biological source

human

biological source

-

biological source

human

assay

≥70% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

specific activity

960-1440 nmol/min·mg

specific activity

-

specific activity

1920-2880 nmol/min·mg

specific activity

182-274 nmol/min·mg

application(s)

cell analysis

application(s)

-

application(s)

-

application(s)

cell analysis

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in E. coli

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

General description

Protein phosphatase 2C-α (PP2C-α), also called protein phosphatase, Mg2+/Mn2+ dependent 1A (PPM1A), is a member of the family of Ser/Thr protein phosphatases and is composed of a catalytic subunit and a constant regulatory subunit. PP2C-α is highly expressed in heart and skeletal muscle. The gene encoding this protein is localized on human chromosome 4q12.

Biochem/physiol Actions

Protein phosphatase 2C-α (PP2C-α) or PPM1A is known to be negative regulators of cell stress response pathways. It has been reported to negatively regulate TGF-β signaling pathway and cell cycle. PP2C-α has been shown to dephosphorylate cyclin-dependent kinases (CDKs) and it may be involved in cell cycle control. It modulates cell migration and invasion. Overexpression of PP2C-α is reported to cause cell-growth arrest or cell death.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Preparation Note

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Integrated analysis of expression and genome alteration reveals putative amplified target genes in esophageal cancer.
Takashi S
Oncology Reports (2007)
The cloning expression and tissue distribution of human PP2C?1
Anna E.Marley
Febs Letters (1998)
PPM1A Methylation Is Associated With Vascular Recurrence in Aspirin-Treated Patients.
Gallego-Fabrega C
Stroke (2016)
Loss of PPM1A expression enhances invasion and the epithelial-to-mesenchymal transition in bladder cancer by activating the TGF-?/Smad signaling pathway.
Geng J
Oncotarget (2014)
Dephosphorylation of Human Cyclin-dependent Kinases by Protein Phosphatase Type 2Ca and ?2 Isoforms*
Aiyang Cheng
The Journal of Biological Chemistry (2000)

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