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T0377

Sigma-Aldrich

Tricine

≥99% (titration)

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Synonym(s):
N-[Tris(hydroxymethyl)methyl]glycine
Linear Formula:
(HOCH2)3CNHCH2CO2H
CAS Number:
Molecular Weight:
179.17
Beilstein/REAXYS Number:
1937804
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

assay

≥99% (titration)

form

crystalline powder

useful pH range

7.4-8.8

pKa (25 °C)

8.1

mp

187 °C

solubility

water: 0.25 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

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This Item
T9784T17917RDD024
Tricine ≥99% (titration)

T0377

Tricine

-
Tricine BioXtra, pH 4.0-6.0 (1 M in H2O), ≥99% (titration)

T9784

Tricine

Premium Grade
Tricine ≥99% (titration), reagent grade, crystalline

T17917

Tricine

Essential Grade
Tricine anhydrous, free-flowing, Redi-Dri™, ≥99%

RDD024

Tricine

-
Quality Level

400

Quality Level

400

Quality Level

200

Quality Level

100

form

crystalline powder

form

powder

form

crystalline

form

powder

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

solubility

water: 0.25 g/mL, clear, colorless

solubility

H2O: 1 M, clear, colorless

solubility

-

solubility

water: 20 % (w/w), clear, colorless

mp

187 °C

mp

-

mp

-

mp

-

General description

Tricine is the most frequently used electrophoresis buffer. It is also useful for the resuspension of cell pellets. Tricine functions as a trailing ion and aids the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems. It has a lower negative charge than glycine, which helps it migrate faster. Tricine has a high ionic strength that allows increased ion movement and less protein movement. This in turn leads to the separation of low molecular weight proteins in lower percent acrylamide gels.

Application

Tricine has been used:
  • to prevent precipitation of salts during autoclaving of Emiliania huxleyi cultures
  • as a component of buffer A for the homogenization of samples like Caenorhabditis elegans, Drosophila, and plants
  • as a component of fresh assay buffer to measure serum melatonin by radioimmunoassay

Buffer component for separation of low molecular weight peptides.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Protocols

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

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