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T5816

Sigma-Aldrich

Tricine

BioPerformance Certified, suitable for cell culture, ≥99% (titration)

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Synonym(s):
2-[[1,3-Dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid, N-[Tris(hydroxymethyl)methyl]glycine, N-[Tris(hydroxymethyl)methyl]glycine
Linear Formula:
(HOCH2)3CNHCH2CO2H
CAS Number:
Molecular Weight:
179.17
Beilstein/REAXYS Number:
1937804
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

grade

BioPerformance Certified

assay

≥99% (titration)

form

crystalline powder

storage condition

dry at room temperature

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and total aerobic microbial count, tested

color

white

useful pH range

7.4-8.8

pKa (25 °C)

8.1

mp

187 °C

cation traces

heavy metals (as Pb): ≤5 ppm

absorption

≤0.02 at 290 at 20%

application(s)

diagnostic assay manufacturing
general analytical
life science and biopharma
sample preparation

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

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General description

Tricine is a zwitterionic biological buffer commonly used in enzyme assays, electrophoresis, and cell culture research. It has a higher negative charge compared to glycine, which enables it to migrate more rapidly. Tricine′s high ionic strength has the ability to cause more ion movement and less protein movement, resulting in the separation of low molecular weight proteins in lower percent acrylamide gels. It has been observed that Tricine is efficient in the separation of proteins ranging from 1 to 100 kDa through electrophoresis. According to a study, the 25 mmol/L Tricine buffer proved to be the most effective among ten other buffers tested for ATP assays using firefly luciferase. Further, Tricine is also used in capillary zone electrophoresis, high performance liquid chromatography and ion exchange chromatography.

Application

Tricine serves as a versatile buffering agent suitable for separating low molecular weight proteins in lower percent acrylamide gels, has demonstrated efficacy in ATP assays employing firefly luciferase, and has shown potential in scavenging hydroxyl radicals during the investigation of radiation-induced membrane damage. Additionally, it has played a role in isolating and purifying ribosomes engaged in the translation of specific mRNA and containing specific peptidyl-tRNA molecules. Tricine has also been employed in the synthesis of RNA oligonucleotides and the fabrication of high-density DNA microarrays, enabling the exploration of mechanisms underlying nucleic acid·ligand interactions and library preparation through photolithography.
Buffer component for separation of low molecular weight peptides.

Features and Benefits

  • Suitable as a Buffer component, for Electrophoresis and Protein separation
  • Effective Buffering from pH 7.4-8.8 (25 °C) with a pKa of 8.1 (25 °C)
  • Tested for Endotoxins and Total Aerobic Microbial Count
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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