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T3824

Sigma-Aldrich

Tyrosinase from mushroom

lyophilized powder, ≥1000 unit/mg solid

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Synonym(s):
Catechol Oxidase, Monophenol Monooxygenase, Monophenol, dihydroxyphenylalanine:oxygen oxidoreductase, Polyphenol Oxidase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

biological source

fungus (mushroom)

form

lyophilized powder

specific activity

≥1000 unit/mg solid

mol wt

119.5 kDa by electrophoresis
128 kDa by sedimentation velocity diffusion
133 kDa by LS

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

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vibrant-m

T3824

Tyrosinase from mushroom

biological source

fungus (mushroom)

biological source

fungus (Trichoderma viride)

biological source

-

biological source

fungus (Dactylium dendroides)

mol wt

119.5 kDa by electrophoresis, 133 kDa by LS, 128 kDa by sedimentation velocity diffusion

mol wt

112 kDa

mol wt

160 kDa

mol wt

-

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

diagnostic assay manufacturing

application(s)

-

General description

Isoelectric point (pI): 4.7-5
pH optimum is 6-7

Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.

Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. The enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper. The copper is probably Cu(I), with inactivation involving oxidation to Cu(II) ion.
Tyrosinase is a copper-containing oxidase with activity for catechols and cresol. Tyrosinase participates in the conversion of tyrosine to dihydroxyphenylalanine (DOPA), and polymerization of DOPA leads to melanin formation. Tyrosinase has a role in browning reactions. The enzyme is said to have two binding sites for aromatic substrates and a distinct binding site for oxygen-copper.

Application

Tyrosinase activity has been assessed in a study that developed an alternative therapeutic agent for treating hyperpigmentation. Tyrosinase has also been used in a study to investigate the oculocutaneous albinism phenotype in the Pakistani population.

Biochem/physiol Actions

Converts tyrosine to L-DOPA or tyramine to dopamine

Physical properties

Isoelectric point (pI): 4.7-5
pH optimum is 6-7

Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.

Unit Definition

One unit = ΔA280 of 0.001 per min at pH 6.5 at 25 °C in 3 mL reaction mix containing L-tyrosine.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Tyrosinase inhibitory effects and inhibition mechanisms of nobiletin and hesperidin from citrus peel crude extracts.
Zhang, et al.
Journal of Enzyme Inhibition and Medicinal Chemistry, 22, 83-90 (2019)
Novel mutations of the tyrosinase (TYR) gene in type I oculocutaneous albinism (OCA1).
R A Spritz et al.
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