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T9265

Sigma-Aldrich

Thyrotropic hormone from human pituitary

lyophilized powder, ≥6.2 IU/mg (immunoassay, non-sterile)

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Synonym(s):
hTSH, TSH, Thyroid stimulating hormone
CAS Number:
MDL number:
NACRES:
NA.32

biological source

human pituitary glands

Quality Level

sterility

non-sterile

form

lyophilized powder

specific activity

≥6.2 IU/mg (immunoassay, non-sterile)

storage condition

(Keep container tightly closed in a dry and well-ventilated place)

technique(s)

cell culture | mammalian: suitable

impurities

HIV-1/HIV-2, tested negative

UniProt accession no.

shipped in

ambient

storage temp.

−20°C

Gene Information

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This Item
T8931P1319E0760
form

lyophilized powder

form

powder

form

powder

form

powder

storage condition

(Keep container tightly closed in a dry and well-ventilated place)

storage condition

-

storage condition

(Keep container tightly closed in a dry and well-ventilated place)

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

activity assay: suitable

technique(s)

cell culture | mammalian: suitable

impurities

HIV-1/HIV-2, tested negative

impurities

-

impurities

22.6-23.8% Nitrogen, 51.9-54.1% carbon

impurities

≤20 EU/vial (LAL test)

UniProt accession no.

P01222, P16473

UniProt accession no.

P01223, P16473

UniProt accession no.

P20396, P21761, P34981

UniProt accession no.

-

General description

Research area: Cell Signaling

Thyrotropic hormone or thyroid stimulating hormone (TSH) belongs to the glycoprotein hormone family. Thyrotroph cells of the anterior pituitary gland produces TSH and is stored in the secretory granules. It comprises a heterodimer of two noncovalently linked subunits, α and β.

Application

Thyrotropic hormone from human pituitary has been used as a supplement in the fetal thyroid organoid culture medium for stimulating maturation. It has also been used as a medium supplement for primary cell culture, and is used further to study the molecular mechanisms of estrogen-mediated growth stimulation of human thyroid tumor cells.

Biochem/physiol Actions

In children, thyroid hormones are very much essential for the normal cognition and neurodevelopment. Thyroid-stimulating hormone (TSH) test is mainly used to diagnose thyroid function.
The product can enhance vascular endothelial growth factor (VEGF) secretion and angiogenesis in thyroid cancer celllines.TSHR activated by TSH activates both the adenosine 3′,5′-cyclic monophosphate (cAMP) pathway (via Gsa) and the phosphoinositol/calcium (IP/Ca2+; via Gq) second messenger signaling pathway by interacting with Gs and Gq G-proteins. In mature adipocytes, TSH aids in lipid metabolism by inhibiting fatty acid synthase (FASN) expression via, activating protein kinase A (PKA)-dependent phosphorylation of extracellular signal-regulated kinases/p42/p44 (ERK1/2), and c-Jun N-terminal kinase (JNK).
Two-chain glycoprotein hormone. The α-chain is not active, biological specificity attributed rather to the β-chain. Activates adenylate cyclase in the thyroid gland, stimulating iodine uptake, thyroxine synthesis and release. Goitrogenic.

Packaging

Package size based on hormone

Physical form

Contains hormone and salts from approx. 0.1 mL of 0.05 M phosphate buffer, pH 7.4.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Thyroid-stimulating hormone
The pituitary, 163-201 (2017)
Impact of light exposure on thyroid-stimulating hormone results using the Siemens Advia Centaur TSH-3Ultra assay
Armer J, et al.
Annals of Clinical Biochemistry, 54(5), 612-615 (2017)
D Manole et al.
The Journal of clinical endocrinology and metabolism, 86(3), 1072-1077 (2001-03-10)
Thyroid tumors are about 3 times more frequent in females than in males. Epidemiological studies suggest that the use of estrogens may contribute to the pathogenesis of thyroid tumors. In a very recent study a direct growth stimulatory effect of
Thyroid-stimulating hormone and thyroid-stimulating hormone receptor structure-function relationships
Szkudlinski MW, et al.
Physiological Reviews, 82(2), 473-502 (2002)
Zhenghao Wu et al.
Journal for immunotherapy of cancer, 10(1) (2022-02-02)
Hormones are identified as key biological variables in tumor immunity. However, previous researches mainly focused on the immune effect of steroid hormones, while the roles that thyroid-stimulating hormone (TSH) played in the antitumor response were far from clear. The source

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