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T9935

Sigma-Aldrich

Trypsin from bovine pancreas

essentially salt-free, lyophilized powder, ≥9,000 BAEE units/mg protein, BioReagent, suitable for cell culture

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Synonym(s):
Serine Protease 1
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

product line

BioReagent

Quality Level

form

essentially salt-free, lyophilized powder

specific activity

≥9,000 BAEE units/mg protein

mol wt

23.8 kDa

composition

protein, ≥80%

technique(s)

cell culture | mammalian: suitable

solubility

hydrochloric acid: soluble 1 mM

storage temp.

−20°C

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Trypsin from bovine pancreas essentially salt-free, lyophilized powder, ≥9,000 BAEE units/mg protein, BioReagent, suitable for cell culture

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Trypsin from bovine pancreas

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T1005

Trypsin from bovine pancreas

Trypsin from bovine pancreas TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein

T8802

Trypsin from bovine pancreas

Trypsin from bovine pancreas TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein

T1426

Trypsin from bovine pancreas

specific activity

≥9,000 BAEE units/mg protein

specific activity

≥6,000 BAEE units/mg protein

specific activity

≥10,000 BAEE units/mg protein

specific activity

≥10,000 BAEE units/mg protein

form

essentially salt-free, lyophilized powder

form

lyophilized powder

form

essentially salt-free, lyophilized powder

form

essentially salt-free, lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

23.8 kDa

mol wt

23.8 kDa

mol wt

23.8 kDa

mol wt

23.8 kDa

solubility

hydrochloric acid: soluble 1 mM

solubility

hydrochloric acid: soluble 1 mM, clear

solubility

hydrochloric acid: soluble 1 mM, clear

solubility

hydrochloric acid: soluble 1 mM

Application

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestions. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.
Trypsin from bovine pancreas has been used for enzymatic digestion of nucleus pulposus (NP) cells during human NP cells isolation. It has also been used as a immunopanning reagent for purification of cells.

Biochem/physiol Actions

Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.

Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.

Components

Trypsin consists of a single chain polypeptide of 223 amino acid residues, produced by the removal of the N-terminal hexapeptide from trypsinogen which is cleaved at the Lys - lle peptide bond. The sequence of amino acids is cross-linked by 6 disulfide bridges. This is the native form of trypsin, beta-trypsin. BETA-trypsin can be autolyzed, cleaving at the Lys - Ser residue, to produce alpha-trypsin. Trypsin is a member of the serine protease family.

Caution

Solutions in 1 mM HCl are stable for 1 year in aliquots and stored at -20°C. The presence of Ca2+ will also diminish the self-autolysis of trypsin and maintain its stability in solution. Trypsin will also retain most of its activity in 2.0 M urea, 2.0 M guanidine HCl, or 0.1% (w/v) SDS.

Unit Definition

One BAEE unit will produce a A253 of 0.001 per minute at pH 7.6 at 25°C using BAEE as a substrate.

Preparation Note

This product is from pancreas sourced from New Zealand. It is soluble in 1 mM HCl at 1 mg/mL.
For applications that involve EDTA, solubilizing trypsin should be done with a buffered salt solution containing no Ca2+ or Mg2+.

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Protocols

Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.

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