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B4875

Sigma-Aldrich

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride

≥98%

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Synonym(s):
Nα-Benzoyl-DL-arginine p-nitroanilide hydrochloride, BANI, BAPNA
Empirical Formula (Hill Notation):
C19H22N6O4 · HCl
CAS Number:
Molecular Weight:
434.88
Beilstein/REAXYS Number:
4081878
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

assay

≥98%

form

powder

color

, white to light yellow to light brown

solubility

DMSO: 50 mg/mL (colorless to dark green-yellow to dark brown-yellow)

storage temp.

−20°C

SMILES string

Cl[H].NC(=N)NCCCC(NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

InChI

1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H

Inchi Key

DEOKFPFLXFNAON-UHFFFAOYSA-N

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This Item
B3279B4750B7632
assay

≥98%

assay

>98% (TLC)

assay

≥98% (TLC)

assay

≥98% (TLC)

solubility

DMSO: 50 mg/mL (colorless to dark green-yellow to dark brown-yellow)

solubility

acetone: water (1:1): 40.00-51.00 mg/mL, clear, colorless to yellow

solubility

DMF: 50 mg/mL, clear, colorless to faintly yellow

solubility

methanol: 20 mg/mL, clear, colorless to light yellow

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

form

powder

form

powder

form

powder

form

powder

color

, white to light yellow to light brown

color

-

color

-

color

-

General description

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride (DL-BAPNA) is a chromogenic substrate for proteolytic enzymes such as trypsin, amidase, and balterobin. Hydrolysis of DL-BAPNA at the bond between the arginine and the p-nitroaniline moieties releases the chromophore p-nitroaniline, which can be detected by colorimetric analysis.

application

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride has been used as a substrate for trypsin and elastase in enzyme analysis.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Substrates

A chromogenic trypsin substrate.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Protocols

Objective: To standardize a procedure for the enzymatic assay of Aprotinin.

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