MilliporeSigma
All Photos(4)

Documents

B3279

Sigma-Aldrich

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride

>98% (TLC)

Sign Into View Organizational & Contract Pricing

Synonym(s):
L-BAPA, L-BAPNA, BANI
Empirical Formula (Hill Notation):
C19H22N6O4 · HCl
CAS Number:
Molecular Weight:
434.88
Beilstein/REAXYS Number:
4081878
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

Quality Level

assay

>98% (TLC)

form

powder

solubility

acetone: water (1:1): 40.00-51.00 mg/mL, clear, colorless to yellow

storage temp.

−20°C

SMILES string

Cl[H].NC(=N)NCCC[C@H](NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

InChI

1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H/t16-;/m0./s1

InChI key

DEOKFPFLXFNAON-NTISSMGPSA-N

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
B3133B4875B4500
solubility

acetone: water (1:1): 40.00-51.00 mg/mL, clear, colorless to yellow

solubility

acetone: water (1:1): 50 mg/mL

solubility

DMSO: 50 mg/mL (colorless to dark green-yellow to dark brown-yellow)

solubility

water: 50 mg/mL, clear, colorless

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

200

form

powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

General description

Na-Benzoyl-L-arginine 4-nitroanilide hydrochloride (L-BAPNA) is a colorless, chromogenic substrate for proteolytic enzymes such as trypsin, actinidin, and papain.

Application

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride has been used as substrate:
  • to measure papain activity in spectrophotometric amidase assay
  • to measure the inhibitory function of human cystatin C (HCC) on the protease activity of papain by colorimetric assay
  • for trypsin

Substrates

Chromogenic substrate for trypsin and other proteolytic enzymes.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

A chemical approach for detecting sulfenic acid-modified proteins in living cells
Reddie KG, et al.
Molecular Biosystems, 4(6), 521- 531 (2008)
Efficient expression, purification and characterization of native human cystatin C in Escherichia coli periplasm
Zhou Y, et al.
Protein Expression and Purification, 111, 18- 22 (2015)
Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom
He YY, et al.
Peptides, 29(10), 1692- 1699 (2008)
Daniele O B Sousa et al.
Nutrients, 7(7), 6038-6054 (2015-07-25)
The biochemical and nutritional attributes of two soybean (Glycine max (L.) Merr.) cultivars, one susceptible (Seridó) and the other resistant (Seridó-RCH) to stem canker, were examined to assess whether the resistance to pathogens was related to levels of antinutritional and/or
Raquel O Rocha et al.
Journal of agricultural and food chemistry, 63(22), 5335-5343 (2015-06-03)
Several studies have described the effects of seed exudates against microorganisms, but only few of them have investigated the proteins that have defensive activity particularly against nematode parasites. This study focused on the proteins released in the exudates of soybean

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service