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B4500

Sigma-Aldrich

Nα-Benzoyl-L-arginine ethyl ester hydrochloride

trypsin substrate

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Synonym(s):
BAEE
Empirical Formula (Hill Notation):
C15H22N4O3 · HCl
CAS Number:
Molecular Weight:
342.82
Beilstein/REAXYS Number:
3781694
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

Quality Level

assay

≥97% (HPLC)

form

powder

solubility

water: 50 mg/mL, clear, colorless

storage temp.

2-8°C

SMILES string

Cl[H].CCOC(=O)[C@H](CCCNC(N)=N)NC(=O)c1ccccc1

InChI

1S/C15H22N4O3.ClH/c1-2-22-14(21)12(9-6-10-18-15(16)17)19-13(20)11-7-4-3-5-8-11;/h3-5,7-8,12H,2,6,9-10H2,1H3,(H,19,20)(H4,16,17,18);1H/t12-;/m0./s1

InChI key

HIXDELXKSSLIKB-YDALLXLXSA-N

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Show Differences

1 of 4

This Item
B3279B3133B4750
solubility

water: 50 mg/mL, clear, colorless

solubility

acetone: water (1:1): 40.00-51.00 mg/mL, clear, colorless to yellow

solubility

acetone: water (1:1): 50 mg/mL

solubility

DMF: 50 mg/mL, clear, colorless to faintly yellow

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

General description

Nα-Benzoyl-L-arginine ethyl ester hydrochloride is a substrate for trypsin. It is a nonprotein substrate used in spectroscopic assays.

Application

  • N

  • α-Benzoyl-L-arginine ethyl ester hydrochloride has been used as a substrate:
  • in peptidyl arginine deiminase (PPAD) assay in P. gingivalis vesicles
  • for assaying proteolytic activity of enzymatic extracts of papaya plant parts
  • in trypsin activity assay of the tail tendon fascicles from rat

Substrates

The prototype substrate for trypsin.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Optima of Trypsin-Catalyzed Hydrolysis and Its Inhibition Determined by SDS-PAGE
Zhou X, et al.
Advances in Enzyme Research, 4(01), 1-1 (2016)
The effect of triclosan on posttranslational modification of proteins through citrullination and carbamylation
Bright R, et al.
Clinical Oral Investigations, 22(1), 487-493 (2018)
Proteolytic activity in enzymatic extracts from Carica papaya L. cv. Maradol harvest by-products
Thomas GE, et al.
Process Biochemistry (Oxford, United Kingdom), 44(1), 77-82 (2009)
Yuchen Li et al.
Nutrients, 12(3) (2020-03-22)
Arthrospira platensis (spirulina) is a cyanobacterium, which contains mainly two phycobiliproteins (PBP), i.e., C-phycocyanin (C-PC) and allophycocyanin (APC). In this study, PBP were hydrolyzed using trypsin, and the composition of the hydrolysate was characterized by HPLC-ESI-MS/MS. Furthermore, the potential anti-diabetic
Paul Kelly et al.
Veterinary research communications, 44(1), 29-39 (2020-02-23)
Isolation and culture of distinct primary endometrial cells are key to reliable in-vitro models to investigate the uterine immune response and optimse new disease interventions. Details on the isolation method and purity of distinct cell populations is lacking in currently

Articles

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

Protocols

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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