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T6424

Sigma-Aldrich

Trypsin from human pancreas

salt-free, lyophilized powder, vial of ≥1,000 BAEE units

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Synonym(s):
Serine Protease 1
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

human pancreas

Quality Level

form

salt-free, lyophilized powder

packaging

vial of ≥1,000 BAEE units

UniProt accession no.

storage temp.

−20°C

Gene Information

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Sigma-Aldrich

T6424

Trypsin from human pancreas

Trypsin from bovine pancreas TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein

T8802

Trypsin from bovine pancreas

Trypsin from porcine pancreas lyophilized powder, BioReagent, suitable for cell culture, 1,000-2,000 BAEE units/mg solid

T4799

Trypsin from porcine pancreas

Trypsin from porcine pancreas tablet, 1 mg tablet

T7168

Trypsin from porcine pancreas

Gene Information

human ... PRSS1(5644), PRSS2(5645), PRSS3(5646)

Gene Information

-

Gene Information

-

Gene Information

-

form

salt-free, lyophilized powder

form

essentially salt-free, lyophilized powder

form

lyophilized powder

form

tablet

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

UniProt accession no.

P07477, P35030, P07478

UniProt accession no.

-

UniProt accession no.

-

UniProt accession no.

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
Trypsin is a serine protease. The pancreas produce the precursor trypsinogen and secretes it to the intestine. Trypsinogen is converted to trypsin by enterokinase in the intestinal mucous membrane.

Application

Trypsin has been used in a study to assess the similarities between the hepatitis E virus and human astrovirus. Trypsin has also been used in a study to characterize a unique technique for culturing primary adult human epithelial progenitor, or stem, cells.

Unit Definition

One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

Quantity

Minimum 1,000 BAEE units/vial

Analysis Note

Protein determined by E1%/280.

Other Notes

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Protocols

Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.

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