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U2258

Sigma-Aldrich

Ubiquitin-7-amido-4-methylcoumarin

solution (supplied in DMSO)

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Synonym(s):
Ubiquitin-AMC
MDL number:
NACRES:
NA.32

assay

≥95% (HPLC)

Quality Level

form

solution (supplied in DMSO)

shipped in

dry ice

storage temp.

−70°C

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A3401C0483B4153
Sigma-Aldrich

U2258

Ubiquitin-7-amido-4-methylcoumarin

Quality Level

100

Quality Level

-

Quality Level

200

Quality Level

-

form

solution (supplied in DMSO)

form

powder

form

solid

form

powder

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

-

storage temp.

−70°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

General description

Manufactured for Sigma by Boston Biochem. Inc.
Ubiquitin-7-amido-4-methylcoumarin (Ub-AMC) is a fluorogenic Ub-base probe. It consists of AMC conjugated to the glycine residue of ubiquitin. Ub-AMC is a fluorogenic substrate for ubiquitin C-terminal hydrolase-L3 (UCH-L3) as well as other UCH isozymes.

Application

Ubiquitin-7-amido-4-methylcoumarin (Ub-AMC) has been used as a fluorogenic substrate in deubiquitinase assay buffer.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

188.6 °F

flash_point_c

87 °C


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Kazunori Hirayama et al.
Bioorganic & medicinal chemistry, 15(21), 6810-6818 (2007-09-01)
UCH-L3 (ubiquitin C-terminal hydrolase-L3) is a de-ubiquitinating enzyme that is a component of the ubiquitin-proteasome system and known to be involved in programmed cell death. A previous study of high-throughput drug screening identified an isatin derivative as a UCH-L3 inhibitor.
Efficient chemical synthesis for the analogue of ubiquitin-based probe Ub--AMC with native bioactivity
Xu L, et al.
Royal Society of Chemistry Advances, 6(53), 47926-47930 (2016)
Sehyoun Yoon et al.
Neuron, 105(3), 506-521 (2019-12-10)
Variants in the ANK3 gene encoding ankyrin-G are associated with neurodevelopmental disorders, including intellectual disability, autism, schizophrenia, and bipolar disorder. However, no upstream regulators of ankyrin-G at synapses are known. Here, we show that ankyrin-G interacts with Usp9X, a neurodevelopmental-disorder-associated deubiquitinase (DUB).

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