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V4888

Sigma-Aldrich

Anti-VSV-G antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-Vesicular Stomatitis Virus glycoprotein
MDL number:
NACRES:
NA.56

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mammals

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 0.5 μg using VSV-G tagged fusion proteins from transfected mammalian cell lysates (tagged fusion proteins from transfected mammalian cell lysates)
indirect immunofluorescence: 1.0 μg/mL using VSV-G tagged fusion proteins in methanol/acetone fixed transiently transfected cells
western blot: 0.1-0.2 μg/mL using VSV-G tagged fusion proteins from transfected mammalian cell lysates

shipped in

dry ice

storage temp.

−20°C

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This Item
SAB4200695C7706A5977
clone

polyclonal

clone

P5D4, monoclonal

clone

P5D4, monoclonal

clone

P5D4, monoclonal

species reactivity

mammals

species reactivity

virus (Vesicular stomatitis virus (VSV))

species reactivity

-

species reactivity

-

conjugate

unconjugated

conjugate

-

conjugate

CY3 conjugate

conjugate

peroxidase conjugate

biological source

rabbit

biological source

mouse

biological source

mouse

biological source

mouse

technique(s)

immunoprecipitation (IP): 0.5 μg using VSV-G tagged fusion proteins from transfected mammalian cell lysates (tagged fusion proteins from transfected mammalian cell lysates), western blot: 0.1-0.2 μg/mL using VSV-G tagged fusion proteins from transfected mammalian cell lysates, indirect immunofluorescence: 1.0 μg/mL using VSV-G tagged fusion proteins in methanol/acetone fixed transiently transfected cells

technique(s)

immunoblotting: 0.5-1 μg/mL using whole extract of human HEK-293T cells over-expressing Vinculin with VSV-G tagged fusion protein., immunocytochemistry: suitable, immunofluorescence: 5-10 μg/mL using COS7 cells over-expressing Vinculin with VSV-G tagged fusion protein., immunoprecipitation (IP): suitable

technique(s)

direct immunofluorescence: 1:10,000 using COS-7 cells transfected with a VSV-G tagged vinculin construct

technique(s)

western blot: 1:1,000 using 20-50 ng of a purified VSV-G tagged fusion protein

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General description

The Anti-VSV-G rabbit antibody reacts with amino- and carboxyl-terminal VSV-G tagged fusion proteins.
Vesicular stomatitis viruses (VSV) belongs to the Vesiculovirus genus of the Rhabdoviridae family. Vesicular stomatitis virus G (VSV G) protein is considered to be the early recognized type III viral fusion protein.

Immunogen

synthetic peptide corresponding to amino acids 497-511 (N-Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys-C) of the Vesicular Stomatitis Virus glycoprotein (VSV-G), conjugated to maleimide activated KLH via a cysteine residue added at the carboxyl terminus. The antibody recognizes the VSV-G tag at both the C-terminus and N-terminus.

Application

Anti-VSV-G antibody produced in rabbit has been used in western blotting and immunoprecipitation.
Antibody is suitable for immunoblotting, immunoprecipitation, and indirect immunofluorescence.
immunocytochemistry.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Western Blotting (1 paper)
Western blotting following immunoprecipitation (1 paper)

Biochem/physiol Actions

Vesicular stomatitis virus G (VSV G) is capable of stimulating cell-cell fusion upon low pH stimulation. The transmembrane region of VSV G protein is highly essential for the virus transition from the hemi-fusion to full fusion state.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Vesicular stomatitis virus G protein transmembrane region is crucial for the hemi-fusion to full fusion transition
Ci Y, et al.
Scientific Reports, 8(1), 10669-10669 (2018)
Vesicular stomatitis virus glycoprotein is a determinant of pathogenesis in swine, a natural host
Martinez I, et al.
Journal of virology, 77(14), 8039-8047 (2003)
Periodic fever with aphthous stomatitis, pharyngitis, and cervical adenitis syndrome is associated with a CARD8 variant unable to bind the NLRP3 inflammasome
Cheung MS, et al.
Journal of immunology (Baltimore, Md. : 1950), 198(5), 2063-2069 (2017)
A mechanism for Wnt coreceptor activation
Tamai K, et al.
Molecular Cell, 13(1), 149-156 (2004)
Igor Červenka et al.
Molecular and cellular biology, 31(1), 179-189 (2010-10-27)
LDL-related protein 6 (LRP6) is a coreceptor of WNTs and a key regulator of the WNT/β-catenin pathway. Upon activation, LRP6 is phosphorylated within its intracellular PPPS/TP motifs. These phosphorylated motifs are required to recruit axin and to inhibit glycogen synthase

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