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V5507

Sigma-Aldrich

Monoclonal Anti-VSV Glycoprotein antibody produced in mouse

clone P5D4, ascites fluid

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Synonym(s):
Monoclonal Anti-VSV Glycoprotein

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

P5D4, monoclonal

contains

15 mM sodium azide

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1:100,000 using whole cell extracts expressing VSV-G tagged fusion protein

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
H1029SAB4200695C7706
clone

P5D4, monoclonal

clone

HIS-1, monoclonal

clone

P5D4, monoclonal

clone

P5D4, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

technique(s)

immunocytochemistry: suitable, western blot: 1:100,000 using whole cell extracts expressing VSV-G tagged fusion protein, immunoprecipitation (IP): suitable

technique(s)

dot blot: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

technique(s)

immunoblotting: 0.5-1 μg/mL using whole extract of human HEK-293T cells over-expressing Vinculin with VSV-G tagged fusion protein., immunocytochemistry: suitable, immunofluorescence: 5-10 μg/mL using COS7 cells over-expressing Vinculin with VSV-G tagged fusion protein., immunoprecipitation (IP): suitable

technique(s)

direct immunofluorescence: 1:10,000 using COS-7 cells transfected with a VSV-G tagged vinculin construct

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

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Specificity

The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain. This antibody has been used for studies on the role of the cytoplasmic domain on newly-synthesized VSV-G during transfer to the plasma membrane and cell surface, using micro-injected antibody, immunoblotting, immunoprecipitation, immunocytochemistry and immunoelectron microscopy. The antibody has been used for the detection, immunoprecipitation and immunocytochemical staining of exogenously introduced constructs tagged with the carboxyl-terminus of VSV-G. This tag does not interfere with the function of the studied protein and can be specifically recognized by the P5D4 antibody without cross-reaction with any endogenous protein.

Immunogen

synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

Storage and Stability

For continuous use, store at 2 °C to 8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Michael C Heymann et al.
Journal of immunology (Baltimore, Md. : 1950), 192(9), 4379-4385 (2014-04-08)
The proinflammatory enzyme caspase-1 plays an important role in the innate immune system and is involved in a variety of inflammatory conditions. Rare naturally occurring human variants of the caspase-1 gene (CASP1) lead to different protein expression and structure and
Lindsey R Robinson et al.
Journal of virology, 90(7), 3640-3649 (2016-01-23)
Endogenous retroviruses (ERVs), the majority of which exist as degraded remnants of ancient viruses, comprise approximately 8% of the human genome. The youngest human ERVs (HERVs) belong to the HERV-K(HML-2) subgroup and were endogenized within the past 1 million years.
Sanne W A Reulen et al.
BMC biotechnology, 9, 66-66 (2009-07-22)
Classical bioconjugation strategies for generating antibody-functionalized nanoparticles are non-specific and typically result in heterogeneous compounds that can be compromised in activity. Expression systems based on self-cleavable intein domains allow the generation of recombinant proteins with a C-terminal thioester, providing a
Samartha G Anekal et al.
Integrative biology : quantitative biosciences from nano to macro, 1(11-12), 664-671 (2009-12-23)
The dynamics of viral infection spread, whether in laboratory cultures or in naturally infected hosts, reflects a coupling of biological and physical processes that remain to be fully elucidated. Biological processes include the kinetics of virus growth in infected cells
Yongxiang Zheng et al.
Nucleic acids research, 43(11), e73-e73 (2015-03-15)
With the aim of broadening the versatility of lentiviral vectors as a tool in nucleic acid research, we expanded the genetic code in the propagation of lentiviral vectors for site-specific incorporation of chemical moieties with unique properties. Through systematic exploration

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