V4889

Anti-phospho-Vinculin (pTyr⁸²²) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-CMD1W, Anti-CMH15, Anti-HEL114, Anti-MV, Anti-MVCL
• MDL number: MFCD06798505
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: human, chicken, rat, mouse
• technique(s): western blot: 1:1,000 using chick embryo fibroblasts (CEFs) transfected with activated Src.
• UniProt accession no.: P18206
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pTyr822)
• Gene Information: human ... VCL(7414) ; mouse ... Vcl(22330) ; rat ... Vcl(305679)

Description

General description

Vinculin (VCL) is a ubiquitously expressed cytoskeletal protein, encoded by the gene mapped to human chromosome 10q11.2-qter. VCL is a vital element of adherens type cell junctions. Vinculin structure contains a globular head connected to a tail domain by a short proline-rich sequence, and an intramolecular interaction between the head and tail hides the various ligand-binding sites in the protein.

Immunogen

synthetic phosphopeptide derived from the region of vinculin that contains tyrosine 822.

Application

Anti-phospho-Vinculin (pTyr822) antibody produced in rabbit has been used in immunofluorescence staining.

Biochem/physiol Actions

Vinculin (VCL) is a cytoskeletal protein that supports cell-to-cell and cell to matrix junctions. It is also essentially involved in normal embryonic development and cardiac function, due to its role in controlling adhesion and motility, as well as cell spreading.In mature focal adhesions (FAs), VCL acts as an important constituent of the “molecular clutch” that induces the transmission of force from cytoplasmic F-actin to membrane-bound integrins. Aberration or mutation of VCL leads to cardiomyopathy, which is associated with hypertension. Experimental studies reveal that, VCL might be used as a biomarker of aging or heart failure. Vinculin associates with FAs and assists in several cellular functions, such as stabilizing and strengthening FAs and promoting their maturation, extracellular matrix (ECM) mechanosensing, regulating actin cytoskeletal dynamics and signaling pathways involved in apoptosis.

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)