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XNABS

Sigma-Aldrich

REDExtract-N-Amp Blood PCR Kit

sufficient for 10 extractions, sufficient for 10 amplifications

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NACRES:
NA.55

usage

sufficient for 10 amplifications
sufficient for 10 extractions
sufficient for 10 reactions

Quality Level

feature

dNTPs included
hotstart

technique(s)

PCR: suitable

color

red

shipped in

wet ice

storage temp.

−20°C

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This Item
XNABXNAB2XNAB2R
usage

sufficient for 10 amplifications

usage

sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions

usage

sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions

usage

sufficient for 1000 amplifications, sufficient for 1000 extractions, sufficient for 1000 reactions

feature

dNTPs included

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

color

red

color

red

color

colorless

color

colorless

XNAB

XNABE, XNABS

XNAB2R, XNAB2RE

XNAB2, XNAB2RE

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

The REDExtract-N-Amp Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR (polymerase chain reaction). This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification. The REDExtract-N-Amp Blood PCR Kit contains a tracking dye that allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

Application

REDExtract-N-Amp Blood PCR Kit has been used to extract genomic DNA from blood samples, peripheral blood leukocytes, parasites and endocervical swab eluates of mice. It has also been used to perform polymerase chain reaction (PCR) using oligonucleotide primers.

Features and Benefits

  • Efficient 8 minute prep allows greater speed and throughput
  • No need for organic extraction, centrifugation or alcohol precipitation
  • Hot start antibody for highly specific PCR amplification of genomic DNA
  • Can be used with whole blood or blood cards
  • Extract stable at 4 °C for at least 6 months
  • Compatible with any format (single tube, 96-well, etc.)
  • Simple, 3 step procedure with no special equipment required
  • REDExtract-N-Amp requires no loading buffers or tracking dyes required for gel analysis
  • PCR products are suitable for direct cloning and sequencing

Principle

Genomic DNA is extracted from 10 μl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • L3289Lysis Solution for BloodSDS

  • N9784Neutralization Solution for BloodSDS

  • P8240REDExtract-N-Amp PCR ReadyMix for BloodSDS

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3


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Sofia Lejon Crottet et al.
Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis, 50(2), 169-174 (2014-04-01)
Starting in 2013, blood donors must be tested at least using: (1) one monoclonal anti-D and one anti-CDE (alternatively full RhCcEe phenotyping), and (2) all RhD negative donors must be tested for RHD exons 5 and 10 plus one further
Olivier A E Sparagano et al.
Annals of the New York Academy of Sciences, 1081, 505-508 (2006-12-01)
Our study is based on the collection of blood and ticks from sheep in Iran and Italy. Polymerase chain reaction (PCR) testing was performed to target the 18S rRNA gene and RLB was performed using previously published probes. In Italy
Kosei Moriyama et al.
Microbiology and immunology, 52(7), 375-382 (2008-08-01)
Bacterial 16S ribosomal RNA genes (rDNA) were detected in blood samples from two healthy individuals by PCR under conditions involving 30 cycles that did not produce any visible products from negative control saline. Even from control samples, PCR involving 35-40
Dongdong Ma et al.
Blood, 118(2), 289-297 (2011-05-19)
HSCs are defined by their ability to self-renew and maintain hematopoiesis throughout the lifespan of an organism. The optical clarity of their embryos and the ease of genetic manipulation make the zebrafish (Danio rerio) an excellent model for studying hematopoiesis.
Slawomir J Teper et al.
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To determine whether gene polymorphisms of the major genetic risk factor for age-related macular susceptibility 2 (ARMS2 A69S) and the complement factor H Y402H influence the response to a variable-dosing treatment regimen with ranibizumab for age-related macular degeneration. This prospective

Articles

Extract-N-Amp is a versatile, combined DNA extraction and amplification kit intended to simplify the generation of PCR products from a range of sample types. This methodology can be used without modification to perform PCR on blood samples stored on FTA blood cards (Whatman) which removes the need for the laborious and costly washing procedures that the standard FTA protocol stipulates. This technique removes the variability between sample preparations and maximizes the number of PCR reactions that can be performed on individual FTA blood card samples.

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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