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Y1250

Sigma-Aldrich

Yeast Nitrogen Base With Amino Acids

Non-selective yeast growth medium

Synonym(s):

ynb with amino acids

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About This Item

UNSPSC Code:
41106212
NACRES:
NA.85

grade

for molecular biology

Quality Level

sterility

non-sterile

form

powder

technique(s)

microbiological culture: suitable

pH

5.3-5.5(0.67% solution)

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.

General description

Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains amino acids, nitrogen, vitamins, trace elements and salts.

Application

Yeast Nitrogen Base With Amino Acids has been used:
  • in the ZYP-31 media preparation for E.coli
  • in synthetic dextrose (SD) medium to evaluate the influence of nitrogen source
  • to culture Candida utilis for the production of 13C-labeled yeast extract as internal standard
  • in the preparation of imaging medium

Suitable for use in classifying yeasts based on carbon requirements. Also suitable for general growth of yeasts with the addition of a carbon source (usually glucose).Yeast nitrogen base with amino acids is a satisfactory nitrogen source for general growth media. Contains all essential nutrients required for the growth of yeasts except carbohydrate.

Components

Component, amount (μg/L unless indicated)

Nitrogen Sources:
Ammonium sulfate, 5.0 g/L

Amino Acids:
L-histidine, 10 mg/L
DL-methionine, 20 mg/L
DL-tryptophan, 20 mg/L

Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400

Trace Elements:
Boric acid
Copper sulfate
Potassium iodide
Ferric chloride
Magnesium sulfate
Sodium molybdate
Zinc sulfate

Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L

Reconstitution

Make a 10x concentrated stock solution by stirring to suspend 6.7g yeast nitrogen base powder and 5 g glucose in 100 mL water. Warm if necessary to aid solubilization. Filter sterilize and store at 2-8°C.

Dilute the 10x concentrated stock to a 1x working solution by adding 100mL concentrated stock to 900mL sterile water.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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Protocols

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

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