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Y1250

Sigma-Aldrich

Yeast Nitrogen Base With Amino Acids

Non-selective yeast growth medium

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Synonym(s):
ynb with amino acids
NACRES:
NA.85

grade

for molecular biology

Quality Level

sterility

non-sterile

form

powder

technique(s)

microbiological culture: suitable

pH

5.3-5.5(0.67% solution)

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.

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Y0626Y1251Y1375
Millipore

Millipore

Y1375

YPD Broth

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

form

powder

form

powder

form

powder

form

powder

technique(s)

microbiological culture: suitable

technique(s)

microbe id | utilization test: suitable, microbiological culture: suitable

technique(s)

-

technique(s)

microbiological culture: suitable

pH

5.3-5.5(0.67% solution)

pH

5.2-5.6(0.67% solution)

pH

-

pH

6.3-6.7

application(s)

microbiology

application(s)

food and beverages
microbiology

application(s)

clinical testing
food and beverages
life science and biopharma, microbiology

application(s)

environmental
food and beverages, microbiology

General description

Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains amino acids, nitrogen, vitamins, trace elements and salts.

Application

Yeast Nitrogen Base With Amino Acids has been used:
  • in the ZYP-31 media preparation for E.coli
  • in synthetic dextrose (SD) medium to evaluate the influence of nitrogen source
  • to culture Candida utilis for the production of 13C-labeled yeast extract as internal standard
  • in the preparation of imaging medium

Suitable for use in classifying yeasts based on carbon requirements. Also suitable for general growth of yeasts with the addition of a carbon source (usually glucose).Yeast nitrogen base with amino acids is a satisfactory nitrogen source for general growth media. Contains all essential nutrients required for the growth of yeasts except carbohydrate.

Components

Component, amount (μg/L unless indicated)

Nitrogen Sources:
Ammonium sulfate, 5.0 g/L

Amino Acids:
L-histidine, 10 mg/L
DL-methionine, 20 mg/L
DL-tryptophan, 20 mg/L

Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400

Trace Elements:
Boric acid
Copper sulfate
Potassium iodide
Ferric chloride
Magnesium sulfate
Sodium molybdate
Zinc sulfate

Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L

Reconstitution

Make a 10x concentrated stock solution by stirring to suspend 6.7g yeast nitrogen base powder and 5 g glucose in 100 mL water. Warm if necessary to aid solubilization. Filter sterilize and store at 2-8°C.

Dilute the 10x concentrated stock to a 1x working solution by adding 100mL concentrated stock to 900mL sterile water.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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