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ZMS1004

Sigma-Aldrich

Anti-Actin Antibody, clone C4 ZooMAb® Mouse Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Synonym(s):
Smooth muscle actin

biological source

mouse

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

C4, recombinant monoclonal
monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 42.05 kDa
observed mol wt ~40 kDa

species reactivity

mouse, rat, human

species reactivity (predicted by homology)

all

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... ACTA2(59)

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Gene Information

human ... ACTA2(59)

Gene Information

human ... ACTB(60)

Gene Information

human ... DAG1(1605)

Gene Information

human ... AIF1(199)

biological source

mouse

biological source

rabbit (recombinant)

biological source

mouse

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

species reactivity

mouse, rat, human

species reactivity

rat, mouse, monkey, human

species reactivity

rabbit, human, mouse

species reactivity

mouse, rat, human

clone

C4, recombinant monoclonal

clone

6L12, monoclonal, recombinant monoclonal

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clone

20A12.1, recombinant monoclonal

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone C4 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects Actin in most vertebrate species. It targets a conserved epitope within 20 amino acids from the N-terminal region.

Immunogen

Purified actin from chicken gizzard.

Application

Anti-Actin, clone C4 ZooMAb, Cat. No. ZMS1004, is a recombinant Mouse monoclonal antibody that targets Actin and is tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Actin in HEK293, HeLa, and NIH3T3 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Actin in rat testis tissue sections.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Actin in rat testis tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Actin (UniProt: P68139) is encoded by the ACTA1 (also known as ACTA) gene (Gene ID: 421534) in chicken. Actins are ubiquitous eukaryotic proteins that serve as multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, transcriptional regulation, and vesicle trafficking. These functions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Beta- and gamma-actins, also known as cytoplasmic actins, are highly conserved in higher animals and are predominantly expressed in non-muscle cells where they control cell structure and motility. They are nearly identical proteins and differ only in four amino acids at the N-terminal region. The other four actin isoforms are typically found in specific adult muscle tissue types. alpha-cardiac and alpha-skeletal actins are expressed in striated cardiac and skeletal muscles, respectively. Alpha and gamma actins are primarily found in vascular smooth muscle and enteric smooth muscles, respectively. Actin cytoskeleton is also involved in the morphogenesis of dendritic spines and is reported to be essential for spine structural stabilization that maintains long-term memory. Clone C4 is prepared as an antibody to chicken gizzard muscles actin, however, it reacts with actins from all vertebrates. It recognizes all six known vertebrate actins and binds to a highly conserved region of actin. This antibody reacts with both globular (G) and filamentous (F) forms of actin and does not interfere with actin polymerization to form filaments. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lessard, DL. (1988). Cell Motil. Cytoskeleton. 10(3); 349-362; Basu, S., and Lamprecht, R. (2018). Front. Mol. Neurosci. 11; 143)..

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1


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Related Content

Choose sustainable options for your research with greener alternative ZooMAb® recombinant antibodies. These BSA-free, carrier-free antibodies are shipped in low-waste packaging and offer maximum stability without compromising performance.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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