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ZMS1043

Sigma-Aldrich

Anti-Bestrophin-1 Antibody, clone E6-6 ZooMAb® Mouse Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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biological source

mouse

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

E6-6, recombinant monoclonal
monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt ~67.68 kDa
observed mol wt ~80 kDa

species reactivity

mouse, human, rabbit

species reactivity (predicted by homology)

monkey, porcine, bovine

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Specificity

Clone E6-6 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects Bestrophin-1. It targets an epitope within 18 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human Bestrophin-1.

Application

Quality Control Testing

Evaluated by Western Blotting in Human retina tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Bestrophin-1 in Human retina tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Bestrophin-1 in Mouse and Rabbit Retina tissue lysates.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Bestrophin-1 in human retina tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Bestrophin-1 in human retina tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Bestrophin-1, clone E6-6 ZooMAb®, Cat. No. ZMS1043, is a recombinant Mouse monoclonal antibody that specifically targets Bestrophin-1 and is tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

Bestrophin-1 (UniProt: O76090; also known as TU15B, Vitelliform macular dystrophy protein 2) is encoded by the BEST1 (also known as VMD2) gene (Gene ID: 7439) in human. Bestrophin-1 is a multi-pass, homopentameric membrane protein that contains three cytoplasmic domains, three extracellular domains, and four transmembrane domains. It is predominantly expressed in the basolateral membrane of the retinal pigment epithelium. However, its expression has also been reported in cortical and hippocampal astrocytes and in cerebellar Bergmann glia and lamellar astrocytes. It serves as a calcium-activated anion channel and its calcium-binding clasp consists of the acidic cluster of amino acids Glu300, Asp301, Asp302, Asp303, and Asp4304). Under physiological conditions, Bestrophin-1 is also involved in glutamate and GABA release from astrocytes to modulate neuronal excitability, synaptic transmission, and synaptic plasticity. Mutations in BEST1 gene have been linked to various macular dystrophies and retinitis pigmentosa that is characterized by retinal pigment deposits and primary loss of photoreceptor cells followed by secondary loss of cone photoreceptors. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Oh, S-J., et al. (2017). Exp. Neurobiol. 26(3); 113-121).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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