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ZRB1026

Sigma-Aldrich

Anti-PD-L1/CD274 Antibody, clone 3O12 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Synonym(s):
B7 homolog 1, B7-H1, CD274, PDCD1 ligand 1, Programmed cell death 1 ligand 1, Programmed death ligand 1

biological source

rabbit (recombinant)

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

3O12, recombinant monoclonal
monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 33.28 kDa
observed mol wt ~42 kDa

species reactivity

human, mouse

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CD274(29126)
mouse ... Cd274(60533)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 3O12 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Programmed cell death 1 ligand 1 (PD-L1). It targets an epitope within the extracellular domain.

Immunogen

His-tagged recombinant fragment corresponding to 218 amino acids from the extracellular region of from human PD-L1.

Application

Anti-PD-L1/CD274, clone 3O12 ZooMAb, Cat. No. ZRB1026, is a recombinant Rabbit monoclonal antibody that targets PD-L1/CD274 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin) and Western Blotting.
Western Blotting Analysis: A 1:100 dilution from a representative lot detected PD-L1/CD274 in mouse placenta tissue lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PD-L1/CD274 in MDA-MB-231 and RAW264.7 cell lines.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PD-L1/CD274 in human placenta and human lung cancer tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound human recombinant PD-L1/CD274 with a KD of 5.5 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Programmed cell death 1 ligand 1 (UniProt Q9NZQ7; also known as B7-H1, B7 homolog 1, CD274, PD-L1, PDCD1 ligand 1, Programmed death ligand 1) is encoded by the CD274 (also known as B7H1, PDCD1L1, PDCD1LG1, PDL1) gene (Gene ID 29126) in human. PD-L1 is a single-pass type I membrane protein that plays a critical role in induction and maintenance of immune tolerance to self. It is expressed on activated T- and B-cells, dendritic cells, keratinocytes, and monocytes. Higher expression has also been reported in heart, skeletal muscle, placenta, and lung. PD-1 and PD-1 ligands 1 and 2 (PD-L1 and PD-L2) are B7:CD28 family members that regulate T cell activation and peripheral tolerance. When engaged together with the TCR, the interaction of PD-1 with its ligands delivers an inhibitory signal to T cell proliferation and cytokine production. While PD-L1 is broadly expressed in hematopoietic and nonhematopoietic cells, PD-L2 expression is highly restricted to antigen presenting cells (APCs), including dendritic cells (DCs) and macrophages. The PD-1 pathway plays a key role in the progressive loss of effector T cell responses during chronic HIV infection. Under some conditions, blockade of this pathway can restore many T cell functions. PD-L1 is initially produced with signal peptide (aa 1-18) sequence, which is subsequently cleaved off to produce the mature protein with a large extracellular (aa 19-238) region that contains an Ig-like V-type domain (aa 19-127) and an Ig-like C2-type domain (aa 133-225), followed by a transmembrane domain (aa 239-259) and a cytoplasmic tail (aa 260-290). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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