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ZRB1180

Sigma-Aldrich

Anti-CD45 Antibody, clone 5L3, ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Synonym(s):
Anti-B220, Anti-CD45, Anti-CD45R, Anti-GP180, Anti-L-CA, Anti-LCA, Anti-LY5, Anti-T200

biological source

rabbit (recombinant)

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

5L3, recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 61.01 kDa
observed mol wt ~200 kDa

species reactivity

rat, human, mouse

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable (peptide)

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... PTPRC(5788)
mouse ... Ptprc(19264)
rat ... Ptprc(24699)

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ZRB1098ZRB1146ZRB1101
clone

5L3, recombinant monoclonal

clone

4L5, recombinant monoclonal

clone

9D14, recombinant monoclonal

clone

4N8, recombinant monoclonal

biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

Gene Information

human ... PTPRC(5788)
mouse ... Ptprc(19264)
rat ... Ptprc(24699)

Gene Information

human ... POU4F1(5457)

Gene Information

human ... CNN1(1264)

Gene Information

human ... POU5F1(5460)
mouse ... Pou5F1(18999)

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 5L3 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects CD45. It targets an epitope within 16 amino acids from the C-terminal half, cytoplasmic domain.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal, cytoplasmic domain of human CD45.

Application

Anti-CD45, clone 5L3 ZooMAb , Cat. No. ZRB1180, is a highly specific recombinant rabbit monoclonal antibody that detects CD45 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD45 in rat spleen and human tonsil tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD45 in human tonsil and mouse spleen tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD45 in Jurkat cells.

Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.

Target description

Receptor-type tyrosine-protein phosphatase C (UniProt: P08575; also known as EC:3.1.3.48, Leukocyte common antigen, L-CA, T200, CD45) is encoded by the PTPRC (also known as CD45) gene (Gene ID: 5788) in human. CD45 is a single-pass type I membrane protein with tyrosine phosphatase activity. CD45 has been shown to be essential for antigen-induced signaling in T-cells and B-cells. It acts as a positive regulator of T-cell coactivation upon binding to DPP4. It is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to generate the mature form that has a extracellular domain (aa 26-577), a short transmembrane domain (aa 578-598), and a cytoplasmic domain (aa 599-1306). CD45 contains two protein tyrosine phosphatase (PTPase) domains (aa 653-912 and 944-1228) and the first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. It also contains 2 fibronectin type-III domains (aa 391-483 and 484-576). Upon T-cell activation, it recruits and dephosphorylates SKAP1 and FYN. It also dephosphorylates LYN, and thereby modulates its activity. CD45 is expressed at high levels on all hematopoietic cells, however, higher density expression occurs on lymphocytes where about 10% of the surface area may be CD45. Eight isoforms of CD45 have been described that are produced by alternative splicing. However, only isoforms 1,2,3,5, and 7 are detected in thymocytes. Mutations in PTPRC gene have been associated with Multiple Sclerosis. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Mada Ghanem et al.
American journal of physiology. Lung cellular and molecular physiology, 321(5), L847-L858 (2021-09-10)
Increased blood fibrocytes are associated with a poor prognosis in fibrotic lung diseases. We aimed to determine whether the percentage of circulating fibrocytes could be predictive of severity and prognosis during coronavirus disease 2019 (COVID-19) pneumonia. Blood fibrocytes were quantified

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