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ZRB1262

Sigma-Aldrich

Anti-Podocin Antibody, clone 1C10 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Synonym(s):
Nephrosis 2 idiopathic steroid-resistant

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

recombinant monoclonal

description

clone 1C10

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 42.2 kDa
observed mol wt ~42 kDa

purified by

using Protein A

species reactivity

human

species reactivity (predicted by homology)

monkey

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

epitope sequence

C-terminal

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

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recombinant

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1C10 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Podocin. It targets an epitope within 19 amino acids from the C-terminal, cytoplasmic domain.

Immunogen

KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human Podocin.

Application

Quality Control Testing

Evaluated by Western Blotting in Human kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Podocin in Human kidney tissue lysate.

Tested applications


Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Podocin in human kidney tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Podocin peptide with a KD of 4.1 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Podocin in human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Podocin (UniProt: Q9NP85; also known as NPHS2) is encoded by the NPHS2 gene (Gene ID: 7827) in human. Podocin is a member of the stomatin family, which consists of hairpin-like integral membrane proteins with intracellular NH2- and COOH-termini. It is exclusively expressed in the podocytes of fetal and mature kidney glomeruli. It is a raft associated component of the glomerular foot-process membrane where it is localized at the insertion of the slit-diaphragm in podocytes. It plays a role in the regulation of glomerular permeability, acting as a linker between the plasma membrane and the cytoskeleton. Two isoforms of Podocin have been described that are produced by alternative splicing. Isoform 1 is located in cell membrane and peripheral membranes and isoform 2 is present in endoplasmic reticulum. Knock-out mice that lack slit diaphragm die within the first weeks of life due to renal failure. Mutations in NPHS2 gene are linked to the development of a steroid resistant nephrotic syndrome (NPHS2) that is characterized by severe proteinuria, resulting in complications such as hypoalbuminemia, hyperlipidemia, edema, and end-stage renal failure in the first two decades of life. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Volker, LA., et al. (2013). BMC Nephrology. 14; Article number: 102; Caridi, G., et al. (2005). Pediatric Res. 57(5 pt2); 54-61).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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