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Anti-LMX-1 Antibody, clone 4L12, ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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LIM homeobox transcription factor 1-alpha, LIM/homeobox protein 1, LIM/homeobox protein LMX1A

biological source

rabbit (recombinant)

Quality Level


expressed in HEK 293 cells



antibody form

purified antibody

antibody product type

primary antibodies


4L12, monoclonal
recombinant monoclonal

product line

ZooMAb® learn more



mol wt

calculated mol wt 42.80 kDa
observed mol wt ~50 kDa

species reactivity


species reactivity (predicted by homology)



antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation


affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable



UniProt accession no.

greener alternative category

shipped in


storage temp.


target post-translational modification


Gene Information

human ... LMX1A(4009)

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This Item
antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody









biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

Gene Information

human ... LMX1A(4009)

Gene Information

human ... IL4I1(259307)

Gene Information

bovine ... Gab1(540085)
human ... GAB1(2549)

Gene Information

human ... BCL2L11(10018)

species reactivity


species reactivity

human, mouse, rat

species reactivity

bovine, human

species reactivity

rat, human, mouse

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.


Clone 4L12 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects LIM homeobox transcription factor 1-alpha (LMX-1). It targets an epitope within 80 amino acids from the C-terminal region.


GST/His-tagged recombinant fragment correspond to 80 amino acids from the C-terminal region of Hamster LMX-1..


Anti-LMX-1, clone 4L12 ZooMAb, Cat. No. ZRB1373, a recombinant Rabbit monoclonal antibody that targets LMX-1 and is tested in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Immunocytochemistry Analysis: A 1:100-1,000 dilution from a representative lot detected LMX-1 in Neuro2A cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected LMX-1 in mouse embryo and human brain tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound recombinant Human LMX-1 protein with a KD of 1.1 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

LIM homeobox transcription factor 1-alpha (UniProt: Q04650; also known as LIM/homeobox protein LMX1A, LIM/homeobox protein 1, LMX-1, LMX1A) is encoded by the LMX1A (also known as LMX1) gene (Gene ID: 101839559) in hamster. LMX1A is a member of the group of LIM-homeobox-containing genes that encode LIM-homeodomain (LIM-HD) protein. LMX-1 is a transcription factor, is expressed in the dorsal mesenchyme and acts by binding to an A/T-rich sequence. It is induced within the ventral midbrain as a response to early signaling It is required for development of the roof plate and for specification of dorsal cell fates in the CNS and developing vertebrae. LMX-1 is reported to play a significant role in of mesencephalic dopamine neurons (mesDA) differentiation of human embryonic stem cells. It has high potential for stimulating the production mesDA that may have applications in the treatment Parkinson disease. Overexpression of LMX-1 is reported to inhibit tumor growth and metastasis. It is shown to be inactivated in gastric cancers. Its tumor growth inhibition is suggested to be due to its inhibitory effect on c-Myc expression. Serum levels of LMX-1 have been used as an effective biomarker for early detection of gastric cancers. Hamster LMX-1 contains two zinc binding domains (aa 33-92 and 93-154). Its DNA binding domain is localized to amino acids 195-254. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Riddle, RD., et al. (1995). Cell. 83(4); 631-640; Qian, P., et al. (2019). PLoS ONE 14(9); e0221640; Li D., and Li, C. (2019). Medicine. 98(22); e15783).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.


300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids



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Iosif Pediaditakis et al.
Nature communications, 12(1), 5907-5907 (2021-10-10)
Parkinson's disease and related synucleinopathies are characterized by the abnormal accumulation of alpha-synuclein aggregates, loss of dopaminergic neurons, and gliosis of the substantia nigra. Although clinical evidence and in vitro studies indicate disruption of the Blood-Brain Barrier in Parkinson's disease

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