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AB10533

Sigma-Aldrich

Anti-LMX-1 Antibody

serum, from rabbit

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Synonym(s):
LIM homeobox transcription factor 1, alpha, LIM/homeobox protein 1.1, LIM/homeobox protein LMX1A
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat

species reactivity (predicted by homology)

hamster (based on 100% sequence homology)

technique(s)

western blot: suitable

isotype

IgG

GenBank accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

hamster ... Lmx1A(101839559)
mouse ... Lmx1A(110648)
rat ... Lmx1A(289201)

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SAB4501768SAB1412493SAB2101355
Anti-LMX-1 Antibody serum, from rabbit

AB10533

Anti-LMX-1 Antibody

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

rabbit

Quality Level

100

Quality Level

-

Quality Level

-

Quality Level

100

Gene Information

hamster ... Lmx1A(101839559)
mouse ... Lmx1A(110648)
rat ... Lmx1A(289201)

Gene Information

human ... LMX1B(4010)

Gene Information

human ... LHX1(3975)

Gene Information

human ... LMX1A(4009)

technique(s)

western blot: suitable

technique(s)

ELISA: 1:40000, western blot: 1:500-1:1000

technique(s)

indirect ELISA: suitable, western blot: 1-5 μg/mL

technique(s)

western blot: suitable

clone

polyclonal

clone

polyclonal

clone

1C11, monoclonal

clone

polyclonal

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General description

LMX-1 is a transcription factor that belongs to the LIM-homeodomain (LIM-HD) transcription factor family. It is induced within the ventral midbrain as a response to early signaling. LMX-1 is a necessary component for the development of the roof plate and for CNS dorsal cell date specification and the development of vertebrae. It is Expressed in roof plate, an organizational locus found in the dorsal CNS that functions to moderate the differentiation and specification of adjacent neurons through the secretion of signaling molecules such as WNT and BMP. Recent studies have indicated that LMX-1 has high potential for stimulating the production of mesencephalic dopamine neurons(mesDA) for the therapeutic treatment Parkinson disease, a condition characterized by dopaminergic neurotransmission decline resulting from the progression of mesDA neuronal degeneration within the substantia nigra pars compacta (SN).

Specificity

This antibody recognizes LMX-1.

Immunogen

Epitope: C-terminus
GST-tagged recombinant protein corresponding to the C-terminus of hamster LMX-1.

Application

Anti-LMX-1 Antibody is an antibody against LMX-1 for use in WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Neuronal & Glial Markers

Quality

Evaluated by Western Blot in mouse testis tissue lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected LMX-1 on 10 µg of mouse testis tissue lysate.

Target description

~50 kDa observed.

Physical form

Unpurified
Unpurified rabbit polyclonal serum containing 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Mouse testis tissue lysate

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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Yanhan Huang et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 38(23), 5429-5440 (2018-05-18)
LIM-domain containing transcription factors (LIM-TFs) are conserved factors important for embryogenesis. The specificity of these factors in transcriptional regulation is conferred by the complexes that they form with other proteins such as LIM-domain-binding (Ldb) proteins and LIM-domain only (LMO) proteins.
Satoshi Okawa et al.
Nature communications, 9(1), 2595-2595 (2018-07-04)
Single-cell RNA sequencing allows defining molecularly distinct cell subpopulations. However, the identification of specific sets of transcription factors (TFs) that define the identity of these subpopulations remains a challenge. Here we propose that subpopulation identity emerges from the synergistic activity
Parthiv Haldipur et al.
eLife, 6 (2017-01-17)
FOXC1 loss contributes to Dandy-Walker malformation (DWM), a common human cerebellar malformation. Previously, we found that complete Foxc1 loss leads to aberrations in proliferation, neuronal differentiation and migration in the embryonic mouse cerebellum (Haldipur et al., 2014). We now demonstrate
M Diana Neely et al.
Toxicological sciences : an official journal of the Society of Toxicology, 159(2), 366-379 (2017-10-01)
Parkinson's disease (PD) is the result of complex interactions between genetic and environmental factors. Two chemically distinct environmental stressors relevant to PD are the metal manganese and the pesticide rotenone. Both are thought to exert neurotoxicity at least in part
Charles Y Lin et al.
Nature, 530(7588), 57-62 (2016-01-28)
Medulloblastoma is a highly malignant paediatric brain tumour, often inflicting devastating consequences on the developing child. Genomic studies have revealed four distinct molecular subgroups with divergent biology and clinical behaviour. An understanding of the regulatory circuitry governing the transcriptional landscapes

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