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ZRB1625

Sigma-Aldrich

Anti-FAK Antibody, clone 1D14 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Synonym(s):
EC:2.7.10.2, FADK 1, FRNK, Focal adhesion kinase 1, Focal adhesion kinase-related nonkinase, PPP1R71, Protein phosphatase 1 regulatory subunit 71, Protein-tyrosine kinase 2, p125FAK, pp125FAK

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1D14, monoclonal
recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 119.23 kDa
observed mol wt ~120 kDa

species reactivity

human, rat

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... PTK2(5747)

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ZRB1153ZRB1698ZRB1437
recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

clone

1D14, monoclonal, recombinant monoclonal

clone

recombinant monoclonal

clone

recombinant monoclonal

clone

recombinant monoclonal

product line

ZooMAb® learn more

product line

ZooMAb® learn more

product line

ZooMAb® learn more

product line

ZooMAb® learn more

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 1D14 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Focal adhesion kinase 1. It targets an epitope within the N-terminal half.

Immunogen

His-tagged recombinant fragment corresponding to the first 423 amino acids from the N-terminal region of human Focal Adhesion Kinase 1.

Application

Anti-FAK, clone 1D14 ZooMAb, Cat. No. ZRB1625, is a recombinant Rabbit monoclonal antibody that specifically detects FAK and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected human recombinant FAK .

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FAK in human placenta and human tonsil tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected FAK in HUVEC .

Affinity Binding Assay: A representative lot of this antibody bound human recombinant FAK with a KD of 8.5 x 10-7 in an affinity binding assay. .

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Focal adhesion kinase 1 (UniProt: Q05397; also known as EC:2.7.10.2, FADK 1, Focal adhesion kinase-related nonkinase, FRNK, Protein phosphatase 1 regulatory subunit 71, PPP1R71, Protein-tyrosine kinase 2, p125FAK, pp125FAK) is encoded by the PTK2 (also known as FAK, FAK1) gene (Gene ID: 5747) in human. FAK is a non-receptor tyrosine kinase that localizes to focal adhesions and contributes to the processes of integrin-mediated cell spreading and migration. It has two nucleotide binding regions (aa 428-434 and 500-502). Its protein kinase domain is localized to amino acids 422-689. FAK 1 plays a role in in regulating cell proliferation, migration, adhesion, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, and apoptosis. It is also required for early embryonic development and placenta development. FAK is involved in integrin signal transduction and in signaling downstream of numerous growth factor receptors and G-protein coupled receptors. FAK undergoes tyrosine phosphorylated in response to various environmental stimuli. Tyrosine 397 is a major autophosphorylation site for FAK, however, it can also be phosphorylated by other kinases. Phosphorylation at tyrosine 397 promotes interaction with SRC and SRC family members, leading to phosphorylation at tyrosine 576 and 577 and at additional tyrosine residues. Phosphorylation at tyrosine 397 is also shown to be important for interaction with BMX, PIK3R1 and SHC1. Microtubule-induced dephosphorylation at tyrosine 397 is crucial for the induction of focal adhesion disassembly and is catalyzed by protein tyrosine phosphatase non-receptor type 11 (PTPN11). Aberrant expression of FAK1 is reported in cancer cell proliferation, migration and invasion, and metastasis. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1


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