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ZRB1732

Sigma-Aldrich

Anti-PCP4 Antibody, clone 1C4-H2 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Synonym(s):
Calmodulin regulator protein PCP4;Brain-specific polypeptide PEP-19;Purkinje cell protein 4

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1C4-H2, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 6.79 kDa
observed mol wt ~12 kDa

purified by

using Protein A

species reactivity

human, mouse

species reactivity (predicted by homology)

rat, feline, canine, bovine, horse

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

epitope sequence

C-terminal

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... PCP4(5121)

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1C4-H2 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Calmodulin regulator protein PCP4. It targets an epitope within 22 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal, IQ domain of human Calmodulin regulator protein PCP4.

Application

Quality Control Testing

Evaluated by Western Blotting in Mouse Brain tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected PCP4 in Mouse Brain tissue lysate.

Tested applications

Affinity Binding Assay: A representative lot of this antibody bound PCP4 with a KD of 1.8 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected PCP4 in SH-SY5Y cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected PCP4 in human cerebellum tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Calmodulin regulator protein PCP4 (UniProt: P48539; also known as Brain-specific polypeptide PEP-19, Purkinje cell protein 4) is encoded by the PCP4 (also known as PEP19) gene (Gene ID: 5121) in human. Calmodulin (CaM) plays a key role in calcium signaling events and its regulation is an important sically dicomponent of cellular homeostasis. PCP4 is a small IQ motif-containing (aa 39-62), intrinsically disordered protein that functions as a modulator of calcium-binding by CaM. Its acidic region (aa 28-40) is reported to be essential for both modulating calcium-binding to CaM and sensitizing cells to ATP-induced calcium-release. It binds to both calcium-free and calcium-bound forms of CaM, however, its affinity for calcium-bound form is about 50-fold greater. PCP4 is also shown to regulate arrhythmogenicity through a cell-autonomous mechanism within the Purkinje fiber network. It regulates cardiac rhythmicity through modulation of autonomic tone. PEP4 null mice exhibit a dramatic reduction in long term plasticity at synapses between granule cell parallel fibers and Purkinje cells. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kim, EE., et al. (2014). J. Clin. Invest. 124(11); 5027-5036; Wang, X., et al. (2013). J. Biol. Chem. 288(3); 2040-2048).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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