ZRB1780
Anti-mTOR Antibody, clone 4O5 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
4O5, monoclonal
recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt ~288.89 kDa
observed mol wt ~290 kDa
species reactivity
mouse, rat, human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 4O5 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects mTOR. It targets an epitope within 24 amino acids from the C-terminal half.
Immunogen
KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal half of human mTOR.
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected mTOR in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected mTOR in A549, L6, and C2C12 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected mTOR in human testis tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected mTOR in A549 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected mTOR in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected mTOR in A549, L6, and C2C12 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected mTOR in human testis tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected mTOR in A549 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-mTOR, clone 4O5 ZooMAb®, Cat. No. ZRB1780, is a rabbit recombinant monoclonal antibody that specifically targets mTOR and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Serine/threonine-protein kinase mTOR (UniProt: P42345; also known as EC:2.7.11.1, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1) is encoded by the MTOR (also known as FRAP, FRAP1, FRAP2, RAFT1, RAPT1) gene (Gene ID: 2475) in human. mTOR is an evolutionarily conserved serine/threonine kinase that acts as a nutrient sensor and regulates growth factor signaling and cellular metabolism. Its catalytic kinase domain located in the C-terminal region exhibits a strong homology to the catalytic domain of PI 3-kinase and PI 4-kinase. It activates translation initiation in response to both hormonal and nutrient signals by phosphorylating at least two translational modulators, p70S6 kinase 1 (S6K1) and eIF-4E-binding protein 1. Cellular mTOR is composed of two large protein complexes, known as mTORC1 and mTORC2. The mTORC1 complex is sensitive to rapamycin and consists of mTOR, Raptor, and mLST8 and two negative regulators, PRAS40 and DEPTOR. The Raptor unit serves as a scaffolding protein to bridge the interaction between mTOR and its substrates. The mTORC2 complex that is insensitive to rapamycin, contains mTOR, Rictor, Sin1, and mLST8 and the newly identified components Protor, Hsp70 and DEPTOR. This complex plays a role in actin cytoskeleton organization and phosphorylates Akt on Ser473 to fully activate it. Deregulation of multiple elements of the mTOR pathway has been reported in many types of cancers. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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