505005
Discovery® RP-Amide C16 (5 µm) HPLC Columns
L × I.D. 5 cm × 4.6 mm, HPLC Column
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UNSPSC Code:
41115700
eCl@ss:
32110501
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product name
Discovery® RP-AmideC16 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
agency
suitable for USP L60
Quality Level
feature
endcapped
manufacturer/tradename
Discovery®
extent of labeling
11% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
5 cm × 4.6 mm
surface area
200 m2/g
surface coverage
2.6 μmol/m2
impurities
<10 ppm metals
matrix
silica gel, high purity, spherical base material
matrix active group
amide, alkyl phase
particle size
5 μm
pore size
180 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
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Features and Benefits
- Excellent retention and resolution of polar compounds
- Unique selectivity compared to C18
- Excellent peak shape and efficiency
- Less hydrophobic than C18 columns
- Compatible with 100% aqueous mobile phases
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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Annamaria Jakab et al.
Journal of pharmaceutical and biomedical analysis, 27(6), 913-921 (2002-02-12)
Retention parameters of 45 different barbituric acid derivatives were determined on an amide embedded RP silica column (Discovery RP-AmideC16) using non-buffered water-acetonitrile eluent systems. Linear correlation were calculated between the logarithm of the capacity factor and the acetonitrile concentration in
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