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53301-U

Supelco

Ascentis® Express Peptide ES-C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

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eCl@ss:
32110501
NACRES:
SB.52

material

stainless steel column

agency

suitable for USP L1

product line

Ascentis®

feature

endcapped: no

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

≤100 °C temp. range
600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

5 cm × 2.1 mm

surface area

90 m2/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform
superficially porous particle

matrix active group

C18 (octadecyl) phase

particle size

2.7 μm

pore size

160 Å

operating pH range

1-9

separation technique

reversed phase

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This Item
53314-U53304-U53306-U
separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

matrix

Fused-Core particle platform, superficially porous particle

matrix

Fused-Core particle platform, superficially porous particle

matrix

Fused-Core particle platform, superficially porous particle

matrix

Fused-Core particle platform, superficially porous particle

pore size

160 Å

pore size

160 Å

pore size

160 Å

pore size

160 Å

product line

Ascentis®

product line

Ascentis®

product line

Ascentis®

product line

Ascentis®

material

stainless steel column

material

stainless steel column

material

stainless steel column

material

stainless steel column

General description

Ascentis Express Peptide ES-C18 columns are specifically engineered to separate higher molecular weight compounds such as peptides and small proteins. These columns contain advanced Fused-Core particles that have larger pores (160 Å versus 90 Å in standard Ascentis Express), bonded with sterically-protected C18 ligands to provide extra stability (ES) at very low pH (< 1) and high temperatures (up to 100ºC). This greatly expands the application range for Ascentis Express columns.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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25G
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1000309185

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Meiyun Shi et al.
Journal of separation science, 38(8), 1351-1357 (2015-01-30)
The pentapeptide thymopentin (Arg-Lys-Asp-Val-Tyr, RKDVY) corresponds to amino acids 32-36 of the 49 amino acid immunomodulatory polypeptide, thymopoietin, whose biological activity is partially reproduced. Thymopentin is widely used in the clinic and represents a promising target for drug design but
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
C Gröer et al.
Journal of pharmaceutical and biomedical analysis, 100, 393-401 (2014-09-15)
Cytochrome P450 (CYP) enzymes and UDP-glucuronosyltransferases (UGT) are major determinants in the pharmacokinetics of most drugs on the market. To investigate their impact on intestinal and hepatic drug metabolism, we developed and validated quantification methods for nine CYP (CYP1A2, CYP2B6

Articles

Choose Ascentis® Express Peptide ES-C18 U/HPLC Columns based on Fused-Core® technology for fast and efficient separation of high-molecular weight compounds, such as peptides and small proteins. With a 2.7 µm particle size and rigorous testing, these columns offer reliable results and high efficiency.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service