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53509-U

Supelco

Ascentis® Express C8, 2.7 μm Guard Cartridge

2.7 μm particle size, L × I.D. 5 mm × 2.1 mm, pkg of 3 ea

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eCl@ss:
32110501
NACRES:
SB.52

material

stainless steel column

agency

suitable for USP L7

product line

Ascentis®

feature

endcapped

packaging

pkg of 3 ea

extent of labeling

5.4% carbon loading

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

5 mm × 2.1 mm

surface area

135 m2/g

matrix

superficially porous particle

matrix active group

C8 (octyl) phase

particle size

2.7 μm

pore size

90 Å

operating pH

2-9

application(s)

food and beverages

separation technique

reversed phase

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This Item
50395-U53512-U50399-U
matrix active group

C8 (octyl) phase

matrix active group

C8 (octyl) phase

matrix active group

C8 (octyl) phase

matrix active group

C8 (octyl) phase

particle size

2.7 μm

particle size

5 μm

particle size

2.7 μm

particle size

5 μm

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

technique(s)

HPLC: suitable, UHPLC-MS: suitable, UHPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

extent of labeling

5.4% carbon loading

extent of labeling

-

extent of labeling

-

extent of labeling

-

General description

Ascentis Express Guard Columns provide physical (filtration) and chemical protection for costly analytical columns without compromising the very high performance of Ascentis Express columns. These Ascentis Express guard columns are capable of continuous use at pressures up to 9000 psi (600 bar) with only hand-tightening. Guard cartridges are easily replaced without removing the guard column holder from the flow path. The cartridges are packed with Ascentis Express Fused-Core® particles. Order guard column holder separately.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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Sreenivasa Rao Chitturi et al.
Journal of pharmaceutical and biomedical analysis, 55(1), 31-47 (2011-02-15)
This paper proposes a simple and selective RP-HPLC method for the determination of process impurities and degradation products (degradants) of atazanavir sulfate (ATV) drug substance. Chromatographic separation was achieved on Ascentis(®) Express C8, (150mm×4.6mm, 2.7μm) column thermostated at 30°C under
Ugandar Reddy Inugala et al.
Journal of chromatographic science, 51(5), 453-459 (2012-10-13)
This paper describes the development of a rapid, novel, stability-indicating gradient reversed-phase high-performance liquid chromatographic method and associated system suitability parameters for the analysis of naproxcinod in the presence of its related substances and degradents using a quality-by-design approach. All
Luigi Silvestro et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 878(30), 3134-3142 (2010-10-20)
Quantitative methods using LC-MS/MS allow achievement of adequate sensitivity for pharmacokinetic studies with clopidogrel; three such methods, with LLOQs as low as 5 pg/mL, were developed and fully validated according to the well established FDA 2001 guidelines. The chromatographic separations
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 81-82, 126-132 (2013-05-07)
A closed-vessel microwave-assisted extraction (MAE) technique was optimized for the first time for the extraction of polyphenols from raw propolis. The results obtained by means of response surface experimental design methodology showed that the best global response was reached when
Han Young Eom et al.
Journal of chromatography. A, 1217(26), 4347-4354 (2010-05-11)
Saikosaponins are triterpene saponins derived from the roots of Bupleurum falcatum L. (Umbelliferae), which has been traditionally used to treat fever, inflammation, liver diseases, and nephritis. It is difficult to analyze saikosaponins using HPLC-UV due to the lack of chromophores.

Articles

When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column.

Protocols

Determination of the thyroid hormones T4, T3 and rT3 from plasma using an Ascentis Express RP-Amide cartridge to trap the analytes, and a Phenyl-Hexyl column to resolve them.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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