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55261-U

Supelco

HybridSPE®-Phospholipid

Cartridge, bed wt. 30 mg, volume 1 mL, pk of 100

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NACRES:
NB.21

material

PE frit (20 μm)
polypropylene hardware

product line

HybridSPE®

composition

bed wt., 30 mg

packaging

pk of 100

technique(s)

solid phase extraction (SPE): suitable

volume

1 mL

matrix active group

zirconia-based phase

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55267-U55276-U52798-U
HybridSPE®-Phospholipid Cartridge, bed wt. 30 mg, volume 1 mL, pk of 100

Supelco

55261-U

HybridSPE®-Phospholipid

HybridSPE®-Phospholipid Cartridge, bed wt. 500 mg, volume 6 mL, pk of 30

Supelco

55267-U

HybridSPE®-Phospholipid

HybridSPE®-Phospholipid Cartridge, bed wt. 30 mg, volume 1 mL, pk of 200, polypropylene material (hardware), PE frit (20 μm)

Supelco

55276-U

HybridSPE®-Phospholipid

HybridSPE®-Phospholipid Small Volume 96-well Plate, bed wt. 15 mg, volume 0.8 mL, pk of 20

Supelco

52798-U

HybridSPE®-Phospholipid

composition

bed wt., 30 mg

composition

bed wt., 500 mg

composition

bed wt., 30 mg

composition

bed wt., 15 mg

packaging

pk of 100

packaging

pk of 30

packaging

pk of 200

packaging

pk of 20

volume

1 mL

volume

6 mL

volume

1 mL

volume

0.8 mL

matrix active group

zirconia-based phase

matrix active group

zirconia-based phase

matrix active group

zirconia-based phase

matrix active group

zirconia-based phase

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

HybridSPE-Phospholipid technology is a simple and generic sample prep platform designed for the gross level removal of endogenous protein and phospholipid interferences from biological plasma and serum prior to LC-MS or LC-MS/MS analysis. Biological plasma or serum is first subjected to protein precipitation via the addition and mixing of acidified acetonitrile. Precipitated proteins are then removed by centrifugation and the resulting supernatant is loaded on the HybridSPE-Phospholipid 96-well plate or cartridge which acts as a chemical filter that specifically targets the removal of endogenous sample phospholipids. The phospholipid retention mechanism is based on a highly selective Lewis acid-base interaction between the proprietary zirconia ions functionally bonded to the HybridSPE-Phospholipid stationary phase and the phosphate moiety consistent with all phospholipids. The resulting eluent is ready for immediate LC-MS or LC-MS-MS analysis.

The "In-well" and "In-cartridge" precipitation methods are available for the HybridSPE-Phospholipid 96-well version and HybridSPE-Phospholipid Ultra cartridge in which biological plasma/serum is first added to either the well or cartridge, followed by acidified acetonitrile (precipitation agent). After a brief mixing/vortexing step, vacuum is applied. Because the 96-well and Ultra cartridge versions contain a series of low porosity hydrophobic filters/frits, the packed-bed filter/frit assembly acts as a depth filter facilitating the concurrent removal of both phospholipids and precipitated proteins during the extraction process. Standard HybridSPE-Phospholipid cartridges require an "off-line" precipitation method.

Features and Benefits

  • For use in conjunction with off-line protein precipitation.
  • Merges the simplicity of protein precipitation and the selectivity of SPE via the targeted removal of phospholipids
  • Reduce ion-suppression through the complete removal of phospholipids and precipitated proteins
  • 2-3 step generic procedure
  • Minimal to no method development
  • Available in 96-well and 1 mL cartridge dimensions

Legal Information

HybridSPE is a registered trademark of Sigma-Aldrich Co. LLC

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Articles

Vitamin D epi-Metabolites Separated with HybridSPE-Phospholipid

This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.

Ion-Suppression & Phospholipid Contamination

We are presenting an article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.

HybridSPE-Phospholipid Technology

This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.

Protocols

Enrichment of Phospholipids in Biological Samples Using HybridSPE-PL

A simple method to enrich phospholipids from plasma samples, involving a HybridSPE-PPT 96-well plate that both retains phospholipids and removes precipitated proteins.

Related Content

Small Molecule HPLC

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

Chromatograms

application for HPLC

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